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长江流域片国家区试大豆品种分子ID构建及遗传多样性分析
引用本文:何琳,何艳琴,刘业丽,等.长江流域片国家区试大豆品种分子ID构建及遗传多样性分析[J].黑龙江八一农垦大学学报,2014(3):5-9.
作者姓名:何琳  何艳琴  刘业丽  
作者单位:[1]黑龙江省农垦科研育种中心,哈尔滨150090; [2]全国农业技术推广服务中心;,哈尔滨150090; [3]中国农业科学院油料作物研究所,哈尔滨150090;
基金项目:国家高技术研究发展计划“863计划”(2012AA101106);现代农业产业体系建设专项(CARS-04-02A).
摘    要:以长江流域片国家区域试验的45份大豆品种为材料,选择分布在大豆基因组8个连锁群的13对SSR标记进行分子ID构建及遗传多样性分析。结果表明:利用Satt138、Satt514、Satt231、Satt380、Satt442和Satt369这6对引物可以将45个参试大豆品种区分开,并获得唯一的分子ID;参试大豆品种间遗传相似系数为0~1.00,平均相似系数为0.442 4,结果说明长江流域片国家区试大豆品种间遗传同源性较小,差异性较大。

关 键 词:大豆  分子ID  遗传多样性

Molecular ID Establishment and Genetic Diversity Analysis of Soybeans Attending National Regional Test of Yangtze River Basin
Institution:He Lin, He Yanqin, Liu Yeli, Yang Zhonglu, Luan Huaihai, Han Xue, Jiang Hongwei, Liu Chunyan, Hu Guohua (1. Crop Research and Breeding Center of Heilongjiang Land reclamation, Harbin 150090; 2.National Agricultural Technology Extension and Service Center; 3.Institute of Oil Crops, Chinese Academy of Agricultural Sciences)
Abstract:45 soybean varieties attending national regional test of Yangtze river basin were investigated,which analyzed the molecular ID and genetic diversity with 13 SSR primers in 8 linkage groups of soybean genetic map.The results showed that 6 SSR markers including Satt138,Satt514,Satt231,Satt380,Satt442 and Satt369 could be used to identify variation among 45 soybean cultivars,and a unique molecular ID was obtained to distinguish varieties. The genetic similarity coefficients among these varieties ranged from 0 to 1.00 and the mean coefficient was 0.442 4. These results indicated that the genetic homology level of soybean varieties were low and the genetic variation among those varieties was large in the study.
Keywords:soybean  molecular ID  genetic diversity
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