| 玫瑰黄链霉菌Men-myco-93-63及其抗生素高产菌株差异蛋白分析 |
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| 引用本文： | 李亚宁,李爱霞.玫瑰黄链霉菌Men-myco-93-63及其抗生素高产菌株差异蛋白分析[J].农业生物技术学报,2008,16(5). |
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| 作者姓名： | 李亚宁 李爱霞 |
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| 作者单位： | 河北农业大学植物保护学院生防室 河北农业大学植物保护学院 |
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| 摘 要： | 玫瑰黄链霉菌Men-myco-93-63是分离自马铃薯疮痂病自然衰退土壤中的一株拮抗菌。前期研究表明，该菌株及其发酵液对棉花黄萎病菌、瓜类白粉病菌等多种重要的植物病原真菌具有较强的抑制作用，具有良好的生防应用潜力。本文利用蛋白质双向凝胶电泳技术研究了Men-myco-93-63与其抗生素高产菌株D12-3之间的蛋白质表达差异。通过优化条件，建立了一套适合该生防菌的双向电泳体系。通过比较蛋白质图谱的差异，发现了四个差异蛋白，质普分析表明，四个蛋白点分别为聚羟基脂肪酸（PHAs）的包涵体蛋白（PhaP），β-内酰胺酶(Beta-lactamase)，甲基接受趋化蛋白(methyl-accepting chemotaxis protein)和ABC转运体(ABC transporter, ATP-binding protein)，这些蛋白可能与该生防菌的抗性有关.
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| 关 键 词： | 玫瑰黄链霉菌 抗生素高产菌株 二维凝胶电泳 蛋白质质谱分析 |
| 收稿时间： | 2008-1-11 |
| 修稿时间： | 2008-3-4 |
Differential Protein Analysis of Streptomyces Roseoflavus Men-myco-93-63 and It’s High-yield antibiotic Strain |
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| Abstract: | S. roseoflavus Men-myco-93-63 isolated from potato scab decline soil is an antagonistic strain. The strain and it’s fermentation can inhibit many phytopathogenic fungi and control the related important plant disease such as cotton verticillium wilt, cumber powdery mildew, and so on. The total protein of S. roseoflavus Men-myco-93-63 and its high-yield antibiotic strain D12-3 were analyzed by two-dimensional gel electrophoresis. As the results, a modified 2-DE protocol and modified electrophoresis program suitable for Men-myco-93-63 were developed in the study. Four different expression protein spots were isolated and identified by Matrix-Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) mass spectrum. The results show the proteins are PhaP, Beta-lactamase, methyl-accepting chemotaxis protein, and ABC transporter, respectively, which perhaps are related with the biosynthesis of antibiotic of S. roseoflavus Men-myco-93-63. |
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