Efficient embryogenic callus induction and plant regeneration from embryonic axis explants inQuercus acutissima |
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| Authors: | Masanori Okamura Toru Taniguchi Teiji Kondo |
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| Institution: | (1) Forest Tree Breeding Center, 3809-1 Ishi, Juo, 319-1301 Ibaraki, Japan;(2) Present address: Kyushu Regional Breeding Office, Forest Tree Breeding Center, 2320 Suya, Nishigoshi, 861-1102 Kumamoto, Japan |
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| Abstract: | Embryogenic callus ofQuercus acutissima was successfully induced from embryogenic cultures, and plants were regenerated from the callus. The development of the techniques
involved will allow mass propagation and gene transformation in this species. Embryogenic cultures were formed from embryonic
axis explants (i.e., embryos without cotyledons) excised from immature embryos, after culture on Murashige and Skoog (MS) medium containing indolebutyric
acid and benzyladenine. Attempts to induce embryogenic cultures from cotyledon explants were unsuccessful. Embryogenic calli
were induced at high frequency from embryogenic cultures on MS medium containing 2,4-dichlorophenoxyacetic acid. However,
benzyladenine inhibited embryogenic callus formation. Somatic embryo development from embryogenic calli occurred on MS medium
in all of the seven cell lines tested. Germination of somatic embryos was induced on half strength MS medium without plant
growth regulators. Finally, acclimated plants growing in soil were obtained. |
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| Keywords: | acclimatization embryogenic callus plant recovery Quercus acutissima somatic embryo |
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