Characterization of wheat-triticale doubled haploid lines by cytological and biochemical markers |
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| Authors: | X Q Zhang X P Wang JK Jing K Ross H Hu J P Gustafson |
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| Institution: | Institute of Genetics, Chinese Academy of Sciences, Beijing, 100101, P.R. China;USDA-Agricultural Research Service, Plant Genetics Research Unit, and Plant Science Unit, University of Missouri, Columbia, Missouri, 65211, USA |
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| Abstract: | Five wheat-triticale doubled haploid (DH) lines— M08, V209, DH220-14-2, DH696-3-4 and M16 —derived from anther culture of F1s resulting from crosses involving hexaploid or octoploid triticale × hexaploid wheat, were characterized by cytological and biochemical markers. Cytological evidence from genomic in situ hybridization and C-banding indicated that DH lines M08 and V209 (2n= 42) each contained a pair of 1BL/1RS translocation chromosomes. DH220-14-2 (2n= 42) was also a translocated line with two pairs of chromosomes containing small fragments of rye. One of the translocation fragments carried the Sec-1R gene originating from the satellite region of 1RS; the origin of the other one remains unknown. DH696-3-4 (2n= 42) contained a 3D(3R) substitution. In M16 (2n= 44), three pairs of rye chromosomes, 3R, 4R and 6R, were present, 4R as an addition and 3D(3R) and 6D(6R) as substitutions. Biochemical, isozyme and storage protein markers confirmed the cytological conclusions. The advantages of transferring alien chromosomes or chromosome fragments into wheat and creating alien aneuploid lines by anther culture of hybrid F1s are discussed. |
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| Keywords: | Triticum aestivum anther culture biochemical marker C-banding genomic in situ hybridization inter-specific hybridization triticale |
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