| 梅花鹿鹿茸间充质层细胞的体外培养和冷冻保存 |
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| 引用本文： | 李光凤,冯海华,赵丽红,张默涵,成文革,岳占碰.梅花鹿鹿茸间充质层细胞的体外培养和冷冻保存[J].经济动物学报,2008,12(3). |
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| 作者姓名： | 李光凤 冯海华 赵丽红 张默涵 成文革 岳占碰 |
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| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062
2. 吉林省生物研究所,长春,130012 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划) |
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| 摘 要： | 为了研究梅花鹿鹿茸间充质层细胞的生物学特性,取梅花鹿鹿茸生长顶端间充质层组织,分离间充质层细胞进行体外培养及冷冻保存。结果表明:在含10%胎牛血清(FBS)的DMEM培养基中,鹿茸间充质层细胞能进行短期体外培养,培养的细胞呈成纤维细胞样,培养7 d可长至汇合。以含5%二甲基亚砜(DMSO)和10%FBS的DMEM为冻存液,经梯度降温后冻存,间充质层细胞复苏后存活率较高。在4℃条件下,间充质层组织在含10%FBS的DMEM中可保存7 d。
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| 关 键 词： | 梅花鹿 鹿茸 间充质层细胞 体外培养 冷冻保存 |
Culture and Cryopreservation of Antler Mesenchymal Layer Cells of Sika Deer in Vitro |
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| LI Guang-feng,FENG Hai-hua,ZHAO Li-hong,ZHANG Mo-han,CHENG Wen-ge,YUE Zhan-peng.Culture and Cryopreservation of Antler Mesenchymal Layer Cells of Sika Deer in Vitro[J].Journal of Economic Animal,2008,12(3). |
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| Authors: | LI Guang-feng FENG Hai-hua ZHAO Li-hong ZHANG Mo-han CHENG Wen-ge YUE Zhan-peng |
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| Abstract: | To study the biological characteristics of sika deer antler mesenchymal layer cells,the mesenchymal layer of growing tip in sika deer antler was collected and a culture and preservation system in vitro for the mesenchymal layer cells was established.The results showed that the antler mesenchymal layer cells could be cultured in DMEM medium with 10% FBS in vitro.The cultured mesenchymal layer cells were fibroblast-like and merged each other after 7 days.The suitable cryopreserved condition for the mesenchymal layer cells was DMEM medium containing 5%DMSO and 10%FBS with gradient-cooling.The mesenchymal layer could be viable at 4℃ for 7 days in DMEM medium containing 10%FBS. |
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| Keywords: | Sika deer antler mesenchymal layer cells culture in vitro cryopreservation |
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