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| 白术叶片再生植株和丛生芽快繁的研究 | |
| 引用本文： | 陈 娟,潘开文,辜 彬,王进闯,吴越华,万 涛.白术叶片再生植株和丛生芽快繁的研究[J].中国农学通报,2006,22(11):65-65. |
| 作者姓名： | 陈 娟 潘开文 辜 彬 王进闯 吴越华 万 涛 |
| 作者单位： | 1. 四川大学生命科学院,成都,610064;中国科学院成都生物研究所,成都,610041 2. 中国科学院成都生物研究所,成都,610041 3. 四川大学生命科学院,成都,610064 4. 四川省西昌林业技校,西昌,615000 5. 四川省林木种苗站,成都,610081 |
| 基金项目： | 中国科学院-乐山市院地合作项目、中国科学院西部之光联合学者项目、国家科技攻关项目子课题“几种重要经济植物无菌苗生产及病害生物防治技术研究”（2005BA807809LA06、2001BA606A-05-04和2004BA606-05-03）、茂县生态站和瓦属山生态站资助 致谢：本文在完成过程中.毕世荣研究员给予了极大,的指导和支持,特此致谢! |
| 摘 要： | 白术是菊科的一种重要药用植物。为满足白术的药用需求，扩大其资源供应，避免传统开荒育苗种植模式对生态环境的破坏，为白术的大规模工厂化育苗及获取药用次生代谢产物提供有效的途径和方法，笔者以白术叶片为外植体经愈伤组织再生植株和丛生芽增殖两种方式建立了白术无菌苗的快速繁殖技术，分析了不同植物激素种类和浓度对白术叶片再生植株及丛生芽增殖的影响，研究了温度和光照条件对白术叶片愈伤组织褐变的影响。研究结果显示在培养基MS+ 6-BA1 mg/L+NAA0.2 mg/L上白术丛生芽增殖倍数最高，可达4.45倍；以白术的叶片愈伤组织诱导及再生植株的最佳培养基为MS +KT 1mg/L+NAA0.2 mg/L，愈伤组织诱导率可达96.7%；芽分化率为90%。不同的细胞分裂素种类与浓度处理差异显著，在白术叶片的愈伤组织诱导中，KT的效果要优于6-BA，但在丛生芽增殖中则表现出高浓度的6-BA要优于KT。白术叶片愈伤组织继代培养中褐变程度与温度、光照条件有关，1000 lx光照强度，20±0.5℃的温度条件下继代培养能有效控制褐变。 |
| 关 键 词： | 银杏 银杏 脱水 活性氧清除酶 |
| 收稿时间： | 2006-08-15 |
| 修稿时间： | 2006-08-152006-08-28 |
Atractylodes Macrocephala Rapid Propagation by Direct Shoot and Plant Regeneration by Leaf |
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| Chen Juan,Pan Kaiwen,Gu Bin,Wang Jinchuang,Wu Yuehu,Wan Tao.Atractylodes Macrocephala Rapid Propagation by Direct Shoot and Plant Regeneration by Leaf[J].Chinese Agricultural Science Bulletin,2006,22(11):65-65. | |
| Authors: | Chen Juan Pan Kaiwen Gu Bin Wang Jinchuang Wu Yuehu Wan Tao |
| Institution: | Chen Juan1,2,Pan Kaiwen2,Gu Bin1,Wang Jinchuang2,Wu Yuehua3,Wan Tao4 |
| Abstract: | Atractylodes macrocephala is an important medicinal herb belonging to the family compositae. In order to provide efficient pathway for commercial production of Atractylodes macrocephala, obtain medicinal secondary metabolite to meet great demand in herbal medicine, and avoid environment destruction induced by tradition cultivation mode of destroying native vegetation, a method for rapid micropropagation of Atractylodes macrocephala through plant regeneration by leaf and direct shoot multiplication were established in the paper .The effect of different cytokinin and concentration on callus introduction from leaf and direct shoot multiplication and the influence of different light and temperature on subcultured callus browning were investigated. The results showed that the highest rate of direct shoot multiplication (4.55 per bud ) was formed at MS+ 6-BA1 mg/L +NAA0.2 mg/L. The optimal substrate for callus introduction and plant regeneration from leaf was MS +KT 1mg/L+NAA0.2 mg/L where the highest callus introduction (96.7%) and proliferation (90%) were obtained. Different cytokinin and variable concentration had significant differences. The effect of KT on callus induction and proliferation was superior to 6-BA, whereas 6-BA was more efficient than KT at higher concentration of 2mg/L on direct shoot multiplication. Callus browning was related to different treatments of temperature and light, and it was availably controlled when cultured under 1000 lx light intensity and 20±0.5℃ temperature. |
| Keywords: | Atractylodes macrocephala rapid propagation Callus induction of leave Regeneration |
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