| 云岭牛无角性状的分子鉴定与应用 |
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| 引用本文： | 李 常,王 宁,刘玉香,亐开兴,张继才,黄必志,雷初朝.云岭牛无角性状的分子鉴定与应用[J].中国牛业科学,2020,46(3):22-24. |
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| 作者姓名： | 李 常 王 宁 刘玉香 亐开兴 张继才 黄必志 雷初朝 |
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| 作者单位： | 陕西省佳县畜牧技术推广站,陕西 佳县719200;云南省草地动物科学研究院,昆明650212;西北农林科技大学动物科技学院,陕西 杨凌712100 |
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| 基金项目： | 国家科技重大专项;国家肉牛牦牛产业技术体系项目;云南省后备人才培养计划 |
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| 摘 要： | 目的]对云岭牛的无角性状进行分子鉴定,以加快云岭牛无角品系的培育速度。方法]采用PCR扩增与琼脂糖电泳分型的方法。结果]在263头云岭牛中,221头牛的基因型与其无角与有角性状完全一致,分子鉴定成功率为83.03%,还有42头云岭牛(16.97%)不能正确鉴定其无角与有角性状。结论]分子鉴定技术可以有效鉴定云岭牛的无角与有角性状,将缩短云岭牛的无角品系选育进程。
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| 关 键 词： | 云岭牛 无角性状 有角性状 分子鉴定 |
Molecular Identification and Application of Polled Trait in Yunling Cattle |
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| LI Chang,WANG Ning,LIU Yu-xiang,QU Kai-xing,ZHANG Ji-cai,HUANG Bi-zhi,LEI Chu-zhao.Molecular Identification and Application of Polled Trait in Yunling Cattle[J].China Cattle Science,2020,46(3):22-24. |
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| Authors: | LI Chang WANG Ning LIU Yu-xiang QU Kai-xing ZHANG Ji-cai HUANG Bi-zhi LEI Chu-zhao |
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| Institution: | (The Extension Station of Animal Husbandry Technology of Jiaxian County in Shaanxi Province, Jiaxian, Shaanxi 719200;Yunnan Academy of Grassland and Animal Science, Kunming 650212;College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100) |
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| Abstract: | Objective]It will speed up the breeding of polled strain in Yunling cattle through identifying the polled trait of Yunling cattle using molecular genetics technology.Method]PCR amplification and agarose gel electrophores methods were used.Result]Among 263 Yunling samples,the genotypes of 221 Yunling samples were completely consistent with the polled and horned traits,the molecular identification rate was 83.03%.However,the polled and horned traits of 42 Yunling samples(16.97%)cannot be identified correctly.Conclusion]The molecular identification technology is effective to identify the polled and horned traits of Yunling cattle,which will shorten the breeding program of the polled line in Yunling cattle. |
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| Keywords: | Yunling cattle polled trait horned trait molecular identification |
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