| 人参生长发育过程中茎内5种酶活性的初步研究 |
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| 引用本文： | 张惠,赵雨,王思明,刘美辰,李晓华.人参生长发育过程中茎内5种酶活性的初步研究[J].吉林农业大学学报,2011,33(6):658-661. |
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| 作者姓名： | 张惠 赵雨 王思明 刘美辰 李晓华 |
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| 作者单位： | 长春中医药大学研发中心,长春,130117 |
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| 基金项目： | 国家自然科学基金项目(30873371) |
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| 摘 要： | 以五年生人参茎为供试材料,分别于人参展叶期、开花期、结果期、果后参根生长期、枯萎期采集人参茎样品,采用中性缓冲液制备粗酶液,应用分光光度法测定硝酸还原酶(NR)、超氧化物歧化酶(SOD)、谷丙转氨酶(GPT)、葡萄糖磷酸异构酶(GPI)、延胡索酸酶(FUM)的活力.结果表明:人参生长发育过程中,茎内NR和FUM均在展叶...
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| 关 键 词： | 人参茎 硝酸还原酶 超氧化物歧化酶 谷丙转氨酶 葡萄糖磷酸异构酶 延胡索酸酶 |
Preliminary Study on Activity of Five Enzymes in Ginseng Stems during Growth and Development |
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| ZHANG Hui,ZHAO Yu,WANG Si-ming,LIU Mei-chen,LI Xiao-hua.Preliminary Study on Activity of Five Enzymes in Ginseng Stems during Growth and Development[J].Journal of Jilin Agricultural University,2011,33(6):658-661. |
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| Authors: | ZHANG Hui ZHAO Yu WANG Si-ming LIU Mei-chen LI Xiao-hua |
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| Institution: | ZHANG Hui,ZHAO Yu,WANG Si-ming,LIU Mei-chen,LI Xiao-huaResearch and Development Center,Changchun University of Traditional Chinese Medicine,Changchun 130117,China |
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| Abstract: | Using five-year old ginseng stems as materials,changes of nitrae reductase(NR),superoxide dismutase(SOD),glutamic-pyruvic transaminase(GPT),glucose phosphate isomerase(GPI) and fumarase(FUM) activity of ginseng stems during growth and development were studied.Ginseng stem samples were collected respectively at the spreading stage,the flowering stage,fruit setting stage,the root growing after fruit stage and the withering stage.Neutral buffer solution was adopted to extract the enzyme solution of radix ginse... |
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| Keywords: | ginseng stem nitrae reductase superoxide dismutase glutamic-pyruvic transaminase glucose phosphate isomerase fumarase |
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