| 转codA基因提高番茄植株的耐热性 |
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| 引用本文： | 李枝梅,窦海鸥,卫丹丹,孟庆伟,Tony Huihuang CHEN,杨兴洪.转codA基因提高番茄植株的耐热性[J].作物学报,2013,39(11):2046-2054. |
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| 作者姓名： | 李枝梅 窦海鸥 卫丹丹 孟庆伟 Tony Huihuang CHEN 杨兴洪 |
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| 作者单位： | 1山东农业大学生命科学学院 / 作物生物学国家重点实验室，山东泰安 271018; 2 Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(2009CB118500);国家高技术研究发展计划(863计划)项目(2012AA10A309);国家自然科学基金项目(30970229);教育部高校博士点基金(20103702110007)资助 |
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| 摘 要： | 以野生型番茄(cv. Moneymaker)和转codA番茄为材料，用不同温度(25、30、35、40、45和50℃)分别处理2 h，测定叶片净光合速率(Pn)、PSII最大光化学效率(Fv/Fm)、过氧化氢(H2O2)含量、丙二醛(MDA)含量、相对电导率(REC)和抗氧化酶活性等生理指标；42℃高温处理0、3和6 h后，检测热响应基因的表达量以及D1蛋白的含量，研究高温胁迫对上述参数的影响，探讨转codA基因提高番茄叶片耐热性的机制。。结果表明，高温胁迫下，转codA基因番茄叶片Pn和Fv/Fm的抑制程度明显低于野生型，H2O2、MDA的积累量以及REC均低于野生型，而且明显增强了过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)的活性。此外，转codA基因番茄叶片中抗氧化酶基因和热胁迫基因的表达水平均高于野生型，而D1蛋白的降解水平低于野生型。转codA基因番茄体内合成的甜菜碱提高了转基因番茄的耐热性，这与提高和维持较高的抗氧化酶活性、促进热激响应基因的表达及减缓D1蛋白的降解等有关。
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| 关 键 词： | codA基因 番茄 高温胁迫 耐热性 甜菜碱 |
| 收稿时间： | 2013-02-26 |
CodA Transgenic Tomato Plants Enhance Tolerance to High Temperature Stress |
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| LI Zhi-Mei;DOU Hai-Ou;WEI Dan-Dan;MENG Qing-Wei;Tony Huihuang CHEN;YANG Xing-Hong.CodA Transgenic Tomato Plants Enhance Tolerance to High Temperature Stress[J].Acta Agronomica Sinica,2013,39(11):2046-2054. |
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| Authors: | LI Zhi-Mei;DOU Hai-Ou;WEI Dan-Dan;MENG Qing-Wei;Tony Huihuang CHEN;YANG Xing-Hong |
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| Institution: | 1.State Key Laboratory of Crop Biology / College of Life Sciences, Shandong Agricultural University, Tai’an 271018, China;2.Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA |
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| Abstract: | The effects of codA gene on photosynthesis, activities of antioxidative enzymes, the expression of the heat response genes and the accumulation of D1 protein 50℃ respectively for two hours, then net photosynthetic rate (Pn), the maximal efficiency of PSII photochemistry (Fv/Fm), hydrogen peroxide (H2O2) content, malondialdehyde (MDA) content, relative electric conductivity (REC) and activities of antioxidativeenzymes were detected. After 42℃ heat stress for 0, 3, 6 hours, the expressions ofantioxidant enzyme genes and heat stress genes and the accumulation of the D1 protein were also determined. The results demonstrated that under high temperature stress, the inhibition degree of Pn and Fv/Fm in codA transgenic tomato plants was lower than that in wild type plants. The accumulation of H2O2, MDA and REC in codA transgenic tomato plants was less than that in wild type plants. And codA transgenic tomato plants also greatly enhanced the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX). Moreover, the expression levels of antioxidant genes and heat response genes in codA transgenic tomato plants was higher than those in wild type plants and the degradation degree of D1 protein in codA transgenic tomato plants were lower than that in wild type plants. It indicated that codA transgenic tomato plants enhance thermotolerance via maintaining higher activities of antioxidant enzymes, accelerating the expression of heat response genes and reducing the degradation of D1 protein. in tomato leaf under different temperature stresses were investigated to reveal the mechanism of thermotolerance in codA-transgenic tomato plants. The wild type (cv. Moneymaker) and codA transgenic tomato plants were treated with 25, 30, 35, 40, 45, and |
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| Keywords: | codA gene Tomato High temperature stress Thermotolerance Glycinebetaine |
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