| 广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应 |
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| 引用本文： | 于苗苗,戴正元,潘存红,陈夕军,余玲,张晓祥,李育红,肖宁,龚红兵,盛生兰,潘学彪,张洪熙,李爱宏.广谱稻瘟病抗性基因Pigm和Pi2的抗谱差异及与Pi1的互作效应[J].作物学报,2013,39(11):1927-1934. |
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| 作者姓名： | 于苗苗 戴正元 潘存红 陈夕军 余玲 张晓祥 李育红 肖宁 龚红兵 盛生兰 潘学彪 张洪熙 李爱宏 |
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| 作者单位： | 江苏里下河地区农业科学研究所 / 国家水稻改良中心南京分中心扬州试验站，江苏扬州225007；2扬州大学植物功能基因组学教育部重点实验室，江苏扬州225009；3江苏丘陵地区镇江农业科学研究所，江苏句容212400 |
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| 基金项目： | 江苏省自然科学基金项目(BK2011427);江苏省农业科技自主创新资金产业体系类项目(CX(12)1003-4)资助 |
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| 摘 要： | Pigm是Pi2基因簇的等位或紧密连锁基因。本研究构建了4个不同遗传背景下Pigm和Pi2的系列近等基因系，204个菌株苗期接种结果显示其抗性频率均超过70%，但Pigm和Pi2的抗谱重叠度仅为54.4%~65.7%，聚合Pi1/Pigm和Pi1/Pi2杂种的抗性频率均超过90%。穗瘟人工接种及病圃自然诱发鉴定表现与苗期接种一致的发病趋势。农艺性状调查结果显示获得的近等基因系与其轮回亲本基本相似，存在较少的累赘连锁。表明Pigm是一个与Pi2抗谱差异明显的广谱抗性基因，对稻瘟病抗性育种具有重要应用价值。
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| 关 键 词： | 稻瘟病抗性基因 标记辅助选择 近等基因系 抗谱 互作效应 |
| 收稿时间： | 2013-03-21 |
Resistance Spectrum Difference between Two Broad-spectrum Blast Resistance Genes,Pigm and Pi2, and Their Interaction Effect on Pi1 |
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| YU Miao-Miao;DAI Zheng-Yuan;PAN Cun-Hong;CHEN Xi-Jun;YU Ling;ZHANG Xiao-Xiang;LI Yu-Hong;XIAO Ning;GONG Hong-Bing;SHENG Sheng-Lan;PAN Xue-Biao;ZHANG Hong-Xi;LI Ai-Hong.Resistance Spectrum Difference between Two Broad-spectrum Blast Resistance Genes,Pigm and Pi2, and Their Interaction Effect on Pi1[J].Acta Agronomica Sinica,2013,39(11):1927-1934. |
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| Authors: | YU Miao-Miao;DAI Zheng-Yuan;PAN Cun-Hong;CHEN Xi-Jun;YU Ling;ZHANG Xiao-Xiang;LI Yu-Hong;XIAO Ning;GONG Hong-Bing;SHENG Sheng-Lan;PAN Xue-Biao;ZHANG Hong-Xi;LI Ai-Hong |
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| Institution: | 1.Lixiahe Agricultural Research Institute of Jiangsu Province / Yangzhou Station of Nanjing Sub-center of National Rice Improvement Center, Yangzhou 225007, China;2.Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China;3.Zhenjiang Institute of Agricultural Science in Jiangsu Hilly Region, Jurong 212400, China |
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| Abstract: | Pigm, an R gene to rice blast disease, is either allelic or tightly linked to Pi2. In this study, a series of near-isogenic lines (NILs) containing Pigm and Pi2 under four genetic backgrounds of rice cultivars were established, respectively. The results from inoculating with 204 representative races at seedling stage showed that the resistance frequency of NILs carryingPigm and Pi2 all reachedmore than70%, but the overlapping degree of resistance spectrum between them was only 54.4% to 65.7%, and the resistance frequencyof hybrids pyramiding Pi1/Pigm and Pi1/Pi2 was more than 90%. Disease development from artificial inoculation at heading stage and natural induction in disease nursery showed consistent trend with that from inoculation at seedling stage. Agronomic traits of most NILs were similar to those of recurrent parent, indicating that the target region contains few linkage drags. In conclusion, Pigm is a broad-spectrum blast resistance gene possessing significant resistance spectrum difference from Pi2, and holds important value for rice blast breeding. |
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| Keywords: | Blast resistance gene Marker-assisted selection Near-isogenic lines Resistance spectrum Interaction effect |
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