| 刺葡萄愈伤组织的诱导和分化培养 |
| |
| 引用本文： | 廖文雪,石贵玉,韦颖,周影.刺葡萄愈伤组织的诱导和分化培养[J].广西园艺,2009,20(6):3-6. |
| |
| 作者姓名： | 廖文雪 石贵玉 韦颖 周影 |
| |
| 作者单位： | 广西师范大学生命科学学院,广西,桂林,541004 |
| |
| 基金项目： | 广西环境工程与保护评价重点实验室研究基金资助;桂科能0702K027 |
| |
| 摘 要： | 以刺葡萄叶片为材料，在1／2MS培养基上附加不同种类及浓度配比的植物激素，研究叶片愈伤组织的诱导及分化培养。实验结果表明：叶片愈伤组织的诱导较容易，最佳诱导培养基配方为以ZT1．0mg／L＋2，4-D2．0mg／L，诱导率可达100％，光培养条件下刺葡萄叶片愈伤组织的诱导率比暗培养的诱导率高。继代培养愈伤组织能诱导出根，但诱导出芽不易，刺葡萄叶片再生植株较难。增殖培养ZT以1．0mg／L＋NAA0．01mg／L为佳，1周增殖率可达1．292％。
|
| 关 键 词： | 刺葡萄 愈伤组织 诱导 分化 |
Callus of Vitis davidii Foex Induction and Differentiate Culture |
| |
| LIAO Wen-xue,SHI Gui-yu,WEI Ying,ZHOU Ying.Callus of Vitis davidii Foex Induction and Differentiate Culture[J].Guangxi Horticulture,2009,20(6):3-6. |
| |
| Authors: | LIAO Wen-xue SHI Gui-yu WEI Ying ZHOU Ying |
| |
| Institution: | LIAO Wen-xue,SHI Gui-yu,WEI Ying,ZHOU Ying (College of Life Sciences,Guangxi Normal University,Guilin 541004,China) |
| |
| Abstract: | Leaves of Vitis davidii Foex were used as the material and different plant hormones were added at various concentrations into 1/2 MS medium to study callus of induction and differentiation.The results showed that inducing callus from its leaves was very easy.ZT 1.0 mg/L and 2,4-D 2.0 mg/L,or ZT 1.0 mg/L and IBA 0.1~1.0 mg/L was the most suitable substrat for inducing callus from leaves,the inducing rate as high as 100 percent.The induction rate of light training was higher than dark training.The callus subc... |
| |
| Keywords: | Vitis davidii Foex Callus Tissue culture Induction Differentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
