| 柑橘炭疽病的病原鉴定和综合防治措施 |
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| 引用本文： | 张玉洁,张志信,李红超.柑橘炭疽病的病原鉴定和综合防治措施[J].北方园艺,2011(2):156-159. |
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| 作者姓名： | 张玉洁 张志信 李红超 |
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| 作者单位： | 文山学院,生化系,云南,文山,663000;文山州生物资源开发研究中心,云南,文山,663000;文山州生物资源开发研究中心,云南,文山,663000;麻栗坡县董干镇麻栗堡小学,云南,麻栗坡,663609 |
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| 基金项目： | 文山学院横向课题基金资助项目 |
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| 摘 要： | 采集桔园感病柑橘,进行病原菌分离鉴定并研究了其对农药的敏感性、形态学和ITS1-5.8S rDNA-ITS2序列分析。结果表明:病原菌为胶孢炭疽菌（Colletotrichum gloeosporioides）;对11种市售农药都具有敏感性,其中氟硅唑、中生菌素、多抗霉素、丙森锌、氟菌唑、异菌脲6种药物对病菌菌丝生长的抑制活性较高,以氟硅唑最为灵敏,其EC50为0.041μg/mL,丙森锌和中生菌素次之,EC50分别为1.59、4.73μg/mL;氟硅唑和百菌清对孢子的抑制效果较好,丙森锌、佳爽和多抗霉素也有一定的保护作用。根据药敏试验和文献方法制定了综合防治方案。
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| 关 键 词： | 柑橘炭疽病 病原菌 鉴定 药物筛选 防治措施 |
Identification and Prevention Method of Pathogenic Fungi of Citrus Anthracnose |
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| ZHANG Yu-jie,ZHANG Zhi-xin,LI Hong-chao.Identification and Prevention Method of Pathogenic Fungi of Citrus Anthracnose[J].Northern Horticulture,2011(2):156-159. |
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| Authors: | ZHANG Yu-jie ZHANG Zhi-xin LI Hong-chao |
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| Institution: | ZHANG Yu-jie~(1,2),ZHANG Zhi-xin~(1,2),LI Hong-chao~(2,3) (1.Department of Biology and Chemistry,Wenshan College,Wenshan,Yunnan 663000;2.Center for Conservation and Utilization of Bio-Resources of Wenshan,Wenshan,Yunnan 663000;3.Elementary School of Malibao Villege,Malipo,Yunnan 663609) |
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| Abstract: | Pathogenic fungus of Citrus anthracnose was isolated from infected fruit and identified as Colletotrichum gloeosporioides according to its cultural and morphological characters and ITS1-5.8S rDNA-ITS 2 sequence analasis.It showed universal sensitivities to 11 commercially available fungicides.Flusilazole showed the best inhibition effect to mycelial growth,with 50%effective content(EC50) value of 0.041μg/mL（tested on PSA plates）,followed by iprovalicarb（1.59μg/ mL）,Zhongshengmycin（4.73μg/mL）.Flusilazole and chlorothalonil had higher inhibition to spore germination of the pathogen,followed by iprovalicarb,Zhongshengmycin and polyoxin.Applicable prevention and cure measures were made according to sensitivity results and reference data. |
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| Keywords: | citrus anthracnose pathogenic fungi fungicide sensitivities prevention and cure means |
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