| 秦岭野生春兰组织培养过程中的褐化控制研究 |
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| 引用本文： | 王宝宁,张显,宋军阳.秦岭野生春兰组织培养过程中的褐化控制研究[J].北方园艺,2011(5):169-172. |
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| 作者姓名： | 王宝宁 张显 宋军阳 |
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| 作者单位： | 西北农林科技大学林学院,陕西杨凌,712100;西北农林科技大学园艺学院,陕西,杨凌712100 |
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| 基金项目： | 国家林业局社会公益资助项目，陕西省农业攻关资助项目，西安市科技局攻关资助项目 |
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| 摘 要： | 以秦岭野生春兰为材料,选花梗和花萼作为外植体,以1/2MS作为基本培养基,运用L9(34)正交实验研究处理对褐化的影响。结果表明:处理4是控制春兰外植体褐化的最佳的方法,即1/2MS+活性炭(AC)1 g/L+PVP 5 g/L+暗培养10 d。
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| 关 键 词： | 秦岭 野生春兰 组织培养 褐化 外植体 |
Studies on Browning of Regenerated System of Wild Cymbidium goeringii in QinLing |
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| WANG Bao-ning,ZHANG Xian,SONG Jun-yang.Studies on Browning of Regenerated System of Wild Cymbidium goeringii in QinLing[J].Northern Horticulture,2011(5):169-172. |
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| Authors: | WANG Bao-ning ZHANG Xian SONG Jun-yang |
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| Institution: | WANG Bao-ning1,ZHANG Xian2,SONG Jun-yang1(1.College of Forestry,Northwest Agricultural and FoRestry University,Yangling,Shaanxi 712100,2.College of Forestry,Shaanxi 712100) |
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| Abstract: | In this research,peduncle and calyx of Cymbidium goeringii were used as the explants,1/2MS were used as the basic medium,L9(34)orthogonal test were used to examine the impact of treatment on the browning.Polyvinyl Pyrrolidone(PVP),citric acid,activated carbon,and different time of dark cultivation were used to study the effect of the control on orchids browning.The results showed that treatment four was the best to control orchid browning and that was 1/2MS+AC 1 g/L+PVP 5 g/L+10 days cultivation in the dark... |
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| Keywords: | Qinling Cymbidium goeringii tissue culture browning explants |
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