| 血红铆钉菇液体发酵培养基的优化研究 |
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| 引用本文： | 高婵娟,季曼,杨翔华,王战勇.血红铆钉菇液体发酵培养基的优化研究[J].北方园艺,2011(3):191-193. |
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| 作者姓名： | 高婵娟 季曼 杨翔华 王战勇 |
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| 作者单位： | 辽宁石油化工大学,环境与生物工程学院,辽宁,抚顺,113001;辽宁石油化工大学,环境与生物工程学院,辽宁,抚顺,113001;辽宁石油化工大学,环境与生物工程学院,辽宁,抚顺,113001;辽宁石油化工大学,环境与生物工程学院,辽宁,抚顺,113001 |
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| 摘 要： | 采用液体发酵培养血红铆钉菇菌丝体,研究了培养基碳源、氮源、无机离子等因素对菌丝体产量的影响。结果表明:适于血红铆钉菇菌丝体液体发酵的培养基配方为:蔗糖30g/L,蛋白胨3g/L,MgSO4 0.5g/L,K2HPO4 1.5g/L,KH2PO4 0.2g/L,ZnSO4 0.03g/L,MnSO40.03g/L;用此培养基培养所得菌丝体产量可达8.655±0.258g/L,是基础培养基的1.5倍。
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| 关 键 词： | 血红铆钉菇 菌丝体 正交实验 |
Optimization of Liquid Culture Medium of Gomphidius rutilus |
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| GAO Chan-juan,JI Man,YANG Xiang-hua,WANG Zhan-yong.Optimization of Liquid Culture Medium of Gomphidius rutilus[J].Northern Horticulture,2011(3):191-193. |
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| Authors: | GAO Chan-juan JI Man YANG Xiang-hua WANG Zhan-yong |
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| Institution: | GAO Chan-juan,JI Man,YANG Xiang-hua,WANG Zhan-yong (School of Environmental and Biological Engineering,Liaoning Shihua University,Fushun,Liaoning 113001) |
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| Abstract: | Gomphidius rutilus was cultured by liquid fermentatioa Some influence factor,such as carbon source, nitrogen source and inorganic ion etc were studied.According with orthogonal test the optimal culture medium was chosen as follows:sucrose was 30 g/L,peptone was 3 g/L,MgSO4 was 0.5 g/L,K2HPO4 was 1.5 g/L,KH2PO4 was 0.2 g/L,ZnSO4 was 0.03 g/L,and MnSO4 was 0.03 g/L.In these conditions,the biomass of Gomphidius rutilus mycelium can reach（8.655±0.258） g/L,it was 1.5 times as the mycelium biomass of the basal medium. |
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| Keywords: | Gomphidius rutilus mycelium orthogonal test |
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