| 桃离体培养与植株再生的优化研究 |
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| 引用本文： | 尚霄丽,汪妮,李明泽,张建鹏,李靖.桃离体培养与植株再生的优化研究[J].北方园艺,2011(3):163-165. |
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| 作者姓名： | 尚霄丽 汪妮 李明泽 张建鹏 李靖 |
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| 作者单位： | 河南农业大学,河南,郑州,450002;濮阳职业技术学院,河南,濮阳,457000;濮阳职业技术学院,河南,濮阳,457000;河南心连心化肥有限公司,河南,新乡,453731;河南农业大学,河南,郑州,450002 |
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| 摘 要： | 以桃杂种单株‘2-7’带腋芽茎段为试材,对桃离体培养进行优化。结果表明:MS+1.5mg/L 6-BA+0.3mg/L IBA培养基中腋芽诱导率最高达88.89%;MS+1.5mg/L 6-BA+0.5mg/L IBA培养基增殖系数最高2.37;试管内培养出的健壮新梢,接种于0.5mg/L IBA的1/2MS培养基中10d后转入无生长调节剂的1/2MS培养基中光/暗(12h/12h)周期下生根效果较好,生根率达90.6%。
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| 关 键 词： | 桃 离体培养 植株再生 |
Study on Optimization of the Conditions for in vitro Culture and Plant Regeneration of Peach |
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| SHANG Xiao-li,WANG Ni,LI Ming-ze,ZHANG Jian-peng,Li Jing.Study on Optimization of the Conditions for in vitro Culture and Plant Regeneration of Peach[J].Northern Horticulture,2011(3):163-165. |
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| Authors: | SHANG Xiao-li WANG Ni LI Ming-ze ZHANG Jian-peng Li Jing |
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| Institution: | SHANG Xiao-li~(1,2),WANG Ni~2,LI Ming-ze~2,ZHANG Jian-peng~3,Li Jing~1 (1.Henan Agricultural University,Zhengzhou,Henan 450002,2.Puyang Vocational and Technical College,Puyang,Henan 457000,3.Henan xinlianxin fertilizer limited company,Xinxiang,Henan 453731) |
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| Abstract: | Stem segments with a single bud of peach were used as explants in culture in vitro and for rapid propagatioa The results showed that suitable medium for initial culture,proliferation were MS+1.5 mg/L 6-BA+0.3 mg/L IBA, MS+1.5 mg/L BA+0.5 mg/L IBA In vitro rooting of cultured vigorous shoot of '2-7' was successful in the light all along by using the 1/2MS salts medium that included 0.5 mg/L IBA and transferred to 1/2MS salts medium without IBA |
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| Keywords: | peach tissue culture plant regeneration |
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