| 微型观赏石榴组培快繁技术 |
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| 引用本文： | 王云波,郑扑红.微型观赏石榴组培快繁技术[J].北方园艺,2011(3):153-155. |
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| 作者姓名： | 王云波 郑扑红 |
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| 作者单位： | 昆明学院,农学院,云南,昆明,650213;昆明学院,农学院,云南,昆明,650213 |
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| 摘 要： | 用饱满的微型观赏石榴种子为材料,以MS为基础培养基,添加不同浓度植物生长调节剂(NAA、6-BA)对种子进行无菌萌发试验,并对形成的愈伤组织及试管植株进行快速增殖和生根研究。结果表明:微型观赏石榴种子无菌萌发的适宜配方为MS+6-BA 3.0mg/L+NAA 1.0mg/L+AC 0.3mg/L;最佳增殖配方为MS+6-BA 1.5mg/L+KT 1.5mg/L+NAA 0.5mg/L;最佳生根配方为MS+6-BA 0.5mg/L+IBA 1.5～2.0mg/L+NAA 1.5～2.0mg/L。
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| 关 键 词： | 微型观赏石榴 组培 种子无菌萌发 增殖 生根 |
Tissue Rapid Propagation Technique of Tiny Ornamental Pomegranate |
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| WANG Yun-bo,ZHENG Pu-hong.Tissue Rapid Propagation Technique of Tiny Ornamental Pomegranate[J].Northern Horticulture,2011(3):153-155. |
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| Authors: | WANG Yun-bo ZHENG Pu-hong |
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| Institution: | WANG Yun-bo,ZHENG Pu-hong (Kunming Agricultural College,Kunming,Yunnan 650213) |
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| Abstract: | Used flush tiny pomegranate seeds as materials,took MS as basic medium,and added with different concentration of plant growth regulators(NAA,6-BA) to test sterile germination,carried out rapid proliferation and rooting study on callus formation.The results showed that the suitable formula for ornamental pomegranate seed germination was MS+6-BA 3.0 mg/L+NAA 1.0 mg/L+AC 0.3 mg/L;the best formula for multiplication was MS+ 6-BA 1.5 mg/L+KT 1.5 mg/L+NAA 0.5 mg/L;the best formula for rooting was MS+6-BA 0.5 mg/L... |
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| Keywords: | tiny ornamental pomegranate tissue culture sterile seed germination multiplication rooting |
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