Seed germination and conservation of endangered species from the Italian Alps: Physoplexis comosa and Primula glaucescens |
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| Authors: | Bruno Cerabolini Rossella De Andreis Roberta M Ceriani Simon Pierce Barbara Raimondi |
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| Institution: | Dipartimento di Biologia Strutturale e Funzionale, Università degli Studi dell'Insubria, via J.H. Dunant 3, 21100 Varese, Italy |
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| Abstract: | Physoplexis comosa and Primula glaucescens, endemic to calcareous grasslands of the Lombardy Prealps, northern Italy, are protected under Annex IV of European Community Directive 92/43/EEC. Seed germination dynamics of these species were studied with the aim of producing appropriate germination protocols for use in ex situ conservation. Seeds were collected from Monte Barro (922 m a.s.l.) and for Primula glaucescens from three additional sites in a more central part of its range (1217-2521 m a.s.l.). Physoplexis comosa germinated only in sterile in vitro conditions, in which germination percentages were extremely low without a supply of gibberellic acid (GA3; 1.7%); the optimum GA3 concentration was 100 mg l−1 (> 90% germination), with lower germination at higher doses of 250-500 mg l−1. Primula glaucescens germinated in both sterile and non-sterile conditions and, although total germination was not affected by GA3 concentration, doses of 10-500 mg l−1 acted equally to prompt earlier and more rapid germination. Primula glaucescens from Monte Barro was characterised by slower and poorer germination than from central sites. |
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| Keywords: | Conservation Endemic species Germination Gibberellic acid Phytohormone |
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