| 甜瓜总RNA快速提取 |
| |
| 引用本文： | 赵惠新,李冠,葛娟,李金玉.甜瓜总RNA快速提取[J].种子,2005,24(5):43-45. |
| |
| 作者姓名： | 赵惠新 李冠 葛娟 李金玉 |
| |
| 作者单位： | 1. 新疆大学生命科学与技术学院,乌鲁木齐,830046;新疆师范大学生命与环境科学学院,830054
2. 新疆大学生命科学与技术学院,乌鲁木齐,830046 |
| |
| 摘 要： | 采用改良的Trizol法对甜瓜叶片的总RNA进行了提取,利用琼脂糖凝胶电泳及Quantity One软件分析、紫外分光光度法、RT-PCR及PCR法进行纯度、浓度、RNA完整性检测.结果表明:所提取的RNA完整性好,纯度高,不含DNA污染,为进行RT-PCR及其他分子生物学实验奠定了良好的基础;新鲜叶片比冷冻叶片的总RNA提取容易,结果更稳定;幼叶比老叶的总RNA含量高.
|
| 关 键 词： | 甜瓜 总RNA提取 |
Total RNA of Cucumis Melon Fast Drawing |
| |
| Zhao Huixin,Li Guan,Ge Juan,Li Jinyu.Total RNA of Cucumis Melon Fast Drawing[J].Seed,2005,24(5):43-45. |
| |
| Authors: | Zhao Huixin Li Guan Ge Juan Li Jinyu |
| |
| Abstract: | We adopted modification of Trizol to draw Cucumis melon total RNA. And the results of the agar-agar candy gel electrophoresis, ultraviolet ray photometer and RT-PCR showed that the purity, concentration and integrity of the drawn RNA were all high, and it was almost had little DNA. The high quality of RNA established a good material foundation for the followed RT-PCR experiment. It was more stable and easier to extract Cucumis melon total-RNA with fresh leaves than that with freezing leaves. And the total-RNA content of young leaves was higher than that older leaves. |
| |
| Keywords: | Cucumis melon Total-RNA drawing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
