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Attributes of wheat cultivars for late autumn sowing in genes expression and field estimates
Institution:1. Department of Plant Growth Principles and Experimental Methodology, UTP University of Science and Technology, 20e Kordeckiego St., 85-225 Bydgoszcz, Poland;2. Department of Crop Science, Breeding and Plant Medicine, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic;1. Department of Wood Science, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 613 00 Brno, Czech Republic;2. Global Change Research Institute CAS, Bělidla 4a, 603 00 Brno, Czech Republic;3. Swiss Federal Research Institute WSL, Zuercherstrasse 111, 8903 Birmensdorf, Switzerland;4. Oeschger Centre for Climate Change Research, University of Bern, 3012 Bern, Switzerland;5. DendroLab Brno, Eliá?ova 37, 616 00 Brno, Czech Republic;6. Department of Forest Management, Faculty of Forestry, Technical University in Zvolen, Slovakia;7. Department of Silviculture and Forest Production, National Forest Centre, Zvolen, Slovakia;8. Department of Archaeology, Faculty of Arts, Comenius University in Bratislava, Gondova 2, 814 99 Bratislava, Slovakia;1. The New Zealand Institute for Plant & Food Research Limited, Private Bag 4704, Lincoln, 7608, New Zealand;2. The New Zealand Institute for Plant & Food Research Limited, Private Bag 4442, Palmerston North, New Zealand;3. The University of Auckland, Department of Statistics, Private Bag 92019, Auckland, 1142, New Zealand;1. School of Mathematics and Computing Science, Changsha University of Science and Technology, Changsha, 410114, PR China;2. School of Mathematics and Statistics, Lanzhou University, Lanzhou 730000, PR China;1. Consiglio per la Ricerca e la Sperimentazione in Agricoltura – Centro di Ricerca per la Cerealicoltura (CRA-CER), SS 16 Km 675, 71122 Foggia, Italy;2. Consiglio per la Ricerca e la Sperimentazione in Agricoltura – Unità di Ricerca per i Processi dell''Industria Agroalimentare (C.R.A. – IAA), Via G. Venezian 26, 20133 Milan, Italy;3. Centro Integrato di Ricerca, Università Campus Bio-Medico di Roma, 00128 Rome, Italy;4. Instituto de Botanica, Nucleo de Pesquisa em Sementes, Av. Miguel Stefano 3687, 04301-012 Sao Paulo, Brazil;1. Department of Large Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark;2. Equine pole, Fundamental and Applied Research for Animals and Health (FARAH), Faculty of Veterinary Medicine, University of Liege, Belgium;3. Mälarhöjdens Veterinärpraktik, Hägersten, Sweden;4. Biochemical Genetics Laboratory, Human Genetics, CHU Liege, University of Liege, Belgium;1. Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, 2462, Hungary;2. Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 165 02 Prague 6, Czech Republic;3. Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR & Faculty of Science, Palacký University, 78 371 Olomouc, Czech Republic;4. Festetics Doctoral School, Georgikon Faculty, University of Pannonia, Keszthely, 8360, Hungary
Abstract:Due to the growing interest in Central and Eastern Europe on cropping of wheat in optional late autumn terms, called facultative, genetic research and field evaluation were taken on four spring cultivars: Tybalt (NL), Monsun (DE), Ostka Smolicka (PL) and Bombona (PL), currently being recommended by breeders. The PPD gene analyze, expression level of dehydrine genes (WCS120 and WDHN13) in cooling test, and qPCR for RNA isolation and analyses of WCS120 and WDHN13 gene expression at the BBCH12 stage of wheat were estimated. Molecular analysis of PPD-D1 gene confirmed the presence of photoperiod sensitive allele ppd-D1b in all tested genotypes. The highest level of NRE WCS120 gene was detected in cultivars Tybalt and Bombona. Two-year field experimental study assessed the growth, development and productivity of facultative and spring crops of studied cultivars. Based on our results from field experiments and result of molecular analysis of alleles of PPD-D1 gene, the tested genotypes can be considered as potentially facultative genotypes.
Keywords:Wheat  Facultative genotypes  Molecular analysis  Dehydrine genes  Productivity  Phenology
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