| 金针菇与凤尾菇科间原生质体融合研究 |
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| 引用本文： | 肖在勤,周俊初.金针菇与凤尾菇科间原生质体融合研究[J].食用菌学报,1998,5(1):6-12. |
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| 作者姓名： | 肖在勤 周俊初 |
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| 作者单位： | 四川省农业科学院土壤肥料研究所，四川省农业科学院土壤肥料研究所，四川省农业科学院土壤肥料研究所，四川省农业科学院土壤肥料研究所，四川省农业科学院土壤肥料研究所，华中农业大学生命技术科学院，华中农业大学生命技术科学院，华中农业大学生命技术科学院，华中农业大学生命技术科学院 成都 610066，成都 610066，成都 610066，成都 610066，成都 610066，武汉 430070，武汉 430070，武汉 430070，武汉 430070 |
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| 基金项目： | 四川省科委“八五”重点攻关项目 |
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| 摘 要： | 金针菇与凤尾菇皆属四极生异宗结合食用菌，其双核异核体菌株都具有锁关联合遗传标记本研究以金针菇和凤尾菇的双核异核菌株为亲本，将热灭活的凤尾菇原生质体，以ＰＥＧ为融合剂，在高下，高ＰＨ值条件下，与金针菇原生质体融合，结果从１３２９个再生菌株中选择出６株双亲细胞质和细胞核都融合的无锁状联合菌株，经融合核分裂技术处理后，融合核分裂成为具有锁状联合的双核菌株。
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| 关 键 词： | 金针菇 凤尾菇 遗传标记 原生质体融合 |
Studies on the Fusion of Protoplasts from Flammulina velutipes and Pleurotus sajor-caju |
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| Xiao Zaiqin Tan Wei Peng Weihong Zheng Linyong Long Zhangfu.Studies on the Fusion of Protoplasts from Flammulina velutipes and Pleurotus sajor-caju[J].Acta Edulis Fungi,1998,5(1):6-12. |
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| Authors: | Xiao Zaiqin Tan Wei Peng Weihong Zheng Linyong Long Zhangfu |
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| Abstract: | Flammulina velutipes (Fr. ) Singer and Pleurotus sajor-caju (Fr.) Singer were tetrapolar and heterothallic edible fungi and their hetero-dicaryotic strains, selected as parent strains in this study, were both with clamp connection as genetic marker. F. velutipes protoplast and heat-treated P. sajor-caju protoplast were fused under high concentration of Ca2 and high pH conditions using PEG as fusogen. And 6 uninucleate fusants without clamp connection, with cytoplasm and cell nuclei of parent strains being all fused, were identified from 1329 regenerate strains. Using fused nucleus division technique, the fused nucleus was divided into dikaryotic strain with clamp connection. While fusants containing the same genetic material as the parent strains was proved to be the genuine fusants by RAPD technique. |
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| Keywords: | Flammulina velutipes- Pleurotus sajor-caju Clamp connection Genetic marker Protoplast fusion Nucleus-fusion strain Fused-nucleus division technique RAPD analysis |
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