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The molecular structure features-immune stimulatory activity of arabinoxylans derived from the pentosan faction of wheat flour |
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| Institution: | 1. University of Agriculture Kraków, Department of Carbohydrates Technology, Balicka 122, 30-149 Kraków, Poland;2. University of Vienna, Department of Pharmaceutical Technology and Biopharmaceutics, Vienna, Austria;1. University of Manitoba, Department of Food Science, Winnipeg, Manitoba, Canada R3T 2N2;2. Canadian Grain Commission, Winnipeg, Manitoba, Canada R3C 3G8;3. University of Manitoba, Richardson Centre for Functional Foods & Nutraceuticals, Winnipeg, Manitoba, Canada R3T 2N2;1. School of Food Science and Nutrition, University of Leeds, Leeds, UK;2. School of Healthcare Science, Manchester Metropolitan University, Manchester, UK;3. Department Health Professions, Faculty of Health, Psychology and Social Care, Manchester Metropolitan University, UK;4. Physiology Division, Department of Zoology, Faculty of Science, Beni-Suef University, Egypt;5. Department of Nutrition and Dietetics, Universiti Putra Malaysia, Malaysia;6. Institute of Food Science & Innovation, University of Chester, Chester, UK |
| Abstract: | Recently, the immune stimulation properties of cereal arabinoxylans (AX) have been reported. The aims of this study were to identify the molecular features and potential immune stimulation activities of AX and enzymatic modified arabinoxylan (AXE) from the pentosan fraction of wheat flour.The results of molecular characterization of AX and AXE show that AXE contains a larger portion (85.7%) of low Mw arabinoxylans (≤25 kDa) and has a higher degree of branch substitution (0.81 ± 0.01) compared to AX (49.5% and 0.62 ± 0.02). In in vitro testing, the ability of AX and AXE to stimulate immune cells, as measured by NO2? production by U937 cells and IL-8 secretion by Caco-2 cells were found to be dose–dependent in the range tested (100–5000 μg/mL p ≤ 0.05). AXE showed a greater activity at each concentration (100–5000 μg/mML) than AX (p ≤ 0.05).In conclusion, the greater immune stimulating activity of AXE may be associated with its low Mw (≤25 kDa) and a higher degree of branch substitution (0.81 ± 0.01). |
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