Quinoa (Chenopodium quinoa Willd.) protein hydrolysates with in vitro dipeptidyl peptidase IV (DPP-IV) inhibitory and antioxidant properties期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Quinoa (Chenopodium quinoa Willd.) protein hydrolysates with in vitro dipeptidyl peptidase IV (DPP-IV) inhibitory and antioxidant properties

Institution:	2. Laboratorio de Alimentos Funcionales, Facultad de Ciencia e Ingeniería en Alimentos, Universidad Técnica de Ambato, Av. Los Chasquis y Rio Payamino, Ambato, Ecuador;3. Departamento de Investigación, Universidad Estatal de Bolivar, Campus Laguacoto, Guaranda, Ecuador

Abstract:	The potential of quinoa to act as a source of dipeptidyl peptidase IV (DPP-IV) inhibitory and antioxidant peptides was studied. A quinoa protein isolate (QPI) with a purity of 40.73 ± 0.90% was prepared. The QPI was hydrolysed at 50 °C for 3 h with two enzyme preparations: papain (P) and a microbial papain-like enzyme (PL) to yield quinoa protein hydrolysates (QPHs). The hydrolysates were evaluated for their DPP-IV inhibitory and oxygen radical absorbance capacity (ORAC) activities. Protein hydrolysis was observed in the QPI control, possibly due to the activity of quinoa endogenous proteinases. The QPI control had significantly higher DPP-IV half maximal inhibitory concentrations (IC₅₀) and lower ORAC values than QPH-P and QPH-PL (P < 0.05). Both QPH-P and QPH-PL had similar DPP-IV IC₅₀ and ORAC values. QPH-P had a DPP-IV IC₅₀ value of 0.88 ± 0.05 mg mL^?1 and an ORAC activity of 501.60 ± 77.34 μmol Trolox equivalent (T.E.) g^?1. To our understanding, this is the first study demonstrating the in vitro DPP-IV inhibitory properties of quinoa protein hydrolysates. QPHs may have potential as functional ingredients with serum glucose lowering properties.

Keywords:	Dipeptidyl peptidase IV inhibition Antioxidant Bioactive peptides Quinoa AAPH"} {"#name":"keyword" "$":{"id":"kwrd0035"} "$$":[{"#name":"text" "_":"2 2′-azobis (2-amidinopropane) dihydrochloride ACE"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"angiotensin converting enzyme ACN"} {"#name":"keyword" "$":{"id":"kwrd0055"} "$$":[{"#name":"text" "_":"acetonitrile AN"} {"#name":"keyword" "$":{"id":"kwrd0065"} "$$":[{"#name":"text" "_":"free amino group content ANOVA"} {"#name":"keyword" "$":{"id":"kwrd0075"} "$$":[{"#name":"text" "_":"analysis of variance BCA"} {"#name":"keyword" "$":{"id":"kwrd0085"} "$$":[{"#name":"text" "_":"bicinchoninic acid BSA"} {"#name":"keyword" "$":{"id":"kwrd0095"} "$$":[{"#name":"text" "_":"bovine serum albumin DPPH"} {"#name":"keyword" "$":{"id":"kwrd0105"} "$$":[{"#name":"text" "_":"2 2-diphenyl-1-picrylhydrazyl DPP-IV"} {"#name":"keyword" "$":{"id":"kwrd0115"} "$$":[{"#name":"text" "_":"dipeptidyl peptidase IV DPP-IV PI"} {"#name":"keyword" "$":{"id":"kwrd0125"} "$$":[{"#name":"text" "_":"DPP-IV inhibitory potency index E:S"} {"#name":"keyword" "$":{"id":"kwrd0135"} "$$":[{"#name":"text" "_":"enzyme to substrate ratio GIP"} {"#name":"keyword" "$":{"id":"kwrd0145"} "$$":[{"#name":"text" "_":"glucose dependent insulinotropic polypeptide GLP-1"} {"#name":"keyword" "$":{"id":"kwrd0155"} "$$":[{"#name":"text" "_":"glucagon-like peptide-1 GP-HPLC"} {"#name":"keyword" "$":{"id":"kwrd0165"} "$$":[{"#name":"text" "_":"gel permeation high-performance liquid chromatography HCl"} {"#name":"keyword" "$":{"id":"kwrd0175"} "$$":[{"#name":"text" "_":"hydrochloric acid HPLC"} {"#name":"keyword" "$":{"id":"kwrd0185"} "$$":[{"#name":"text" "_":"high performance liquid chromatography half maximal inhibitory concentration NaOH"} {"#name":"keyword" "$":{"id":"kwrd0205"} "$$":[{"#name":"text" "_":"sodium hydroxide ORAC"} {"#name":"keyword" "$":{"id":"kwrd0215"} "$$":[{"#name":"text" "_":"oxygen radical absorbance capacity P"} {"#name":"keyword" "$":{"id":"kwrd0225"} "$$":[{"#name":"text" "_":"papain PL"} {"#name":"keyword" "$":{"id":"kwrd0235"} "$$":[{"#name":"text" "_":"papain-like enzyme QPH"} {"#name":"keyword" "$":{"id":"kwrd0245"} "$$":[{"#name":"text" "_":"quinoa protein hydrolysate QPH-P"} {"#name":"keyword" "$":{"id":"kwrd0255"} "$$":[{"#name":"text" "_":"QPH obtained with P QPH-PL"} {"#name":"keyword" "$":{"id":"kwrd0265"} "$$":[{"#name":"text" "_":"QPH obtained with the PL QPI"} {"#name":"keyword" "$":{"id":"kwrd0275"} "$$":[{"#name":"text" "_":"quinoa protein isolates RP-HPLC"} {"#name":"keyword" "$":{"id":"kwrd0285"} "$$":[{"#name":"text" "_":"reverse-phase high-performance liquid chromatography RuBisCo"} {"#name":"keyword" "$":{"id":"kwrd0295"} "$$":[{"#name":"text" "_":"ribulose-1 5-bisphosphate carboxylase/oxygenase SD"} {"#name":"keyword" "$":{"id":"kwrd0305"} "$$":[{"#name":"text" "_":"standard deviation SDS-PAGE"} {"#name":"keyword" "$":{"id":"kwrd0315"} "$$":[{"#name":"text" "_":"sodium dodecyl sulphate polyacrylamide gel electrophoresis TFA"} {"#name":"keyword" "$":{"id":"kwrd0325"} "$$":[{"#name":"text" "_":"trifluoroacetic acid TCA"} {"#name":"keyword" "$":{"id":"kwrd0335"} "$$":[{"#name":"text" "_":"trichloroacetic acid T E "} {"#name":"keyword" "$":{"id":"kwrd0345"} "$$":[{"#name":"text" "_":"Trolox equivalent TNBS"} {"#name":"keyword" "$":{"id":"kwrd0355"} "$$":[{"#name":"text" "_":"2 4 6-trinitrobenzenesulfonic acid TRIS"} {"#name":"keyword" "$":{"id":"kwrd0365"} "$$":[{"#name":"text" "_":"tris(hydroxymethyl)aminomethane T2D"} {"#name":"keyword" "$":{"id":"kwrd0375"} "$$":[{"#name":"text" "_":"type 2 diabetes
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