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牡丹栽培品种的RAPD分析
引用本文:陈向明,郑国生,张圣旺.牡丹栽培品种的RAPD分析[J].园艺学报,2001,28(4):370-372.
作者姓名:陈向明  郑国生  张圣旺
作者单位:( 合肥教育学院, 合肥230001 ; 山东农业大学生命科学学院, 泰安271018)
基金项目:山东省良种产业化工程项目
摘    要: 对7 种花色系的35 个牡丹栽培品种进行RAPD 分析, 34 个随机引物共扩增出418 个位点, 其中337 个(80. 62 %) 多态位点表明受试品种间丰富的遗传多态性。同一花色的不同品种间和不同花色系间都存在较大的遗传多态性, 不同花色系间多态性位点频率大小依次排序为蓝色系> 黄色系> 深红色系> 黑色系> 白色系> 绿色系> 红色系; 利用UPGMA 软件进行聚类分析, 35 个品种被分为5 个遗传聚类组, 除红色系外, 其它6 种花色与遗传聚类组的划分无必然相关性。

关 键 词:牡丹  品种  基因组  遗传多样性  RAPD
文章编号:0513-353X(2001)04-0370-03
修稿时间:2000年12月22

RAPD Analysis of Tree Peony Cultivars
Chen Xiangming ,Zheng Guosheng ,and Zhang Shengwang.RAPD Analysis of Tree Peony Cultivars[J].Acta Horticulturae Sinica,2001,28(4):370-372.
Authors:Chen Xiangming  Zheng Guosheng  and Zhang Shengwang
Institution:Department of Cardiology, the Affiliated Hospital of Southeast University, Nanjing 210009, China
Abstract:The genomic diversity of thirty five tree peony cultivars of 7 different flower color were analised by RAPD.The results showed that 337(80.62%)polymorphic sites were detected among the total 418 sites amplified indicating abundant genomic diversity of tree peony cultivars.Similar results were also revealed between accessions within the same flower color series,but significant differences were observed between different flower color series in the order as Blue series>Yellow>Dark red>Black>White>Green>Red ones based upon the percentage of the polymorphic sites.The genetic distance and genetic relationships of the accessions were constructed through unweighted paired group method with arithmetic averages cluster analysis,all accessions were grouped into 5 genetic types,no significant correlation between flower color series and genetic types were detected except Red flower series.
Keywords:Tree peony cultivar  Genome  Genetic diversity  RAPD  
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