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牛DGAT1基因K232A取代对3个品种奶牛群体部分经济性状的影响
引用本文:徐秀容,高 雪,许尚忠.牛DGAT1基因K232A取代对3个品种奶牛群体部分经济性状的影响[J].西北农林科技大学学报(社会科学版),2006,34(9):6-10.
作者姓名:徐秀容  高 雪  许尚忠
作者单位:1. 西北农林科技大学,动物科技学院,陕西,杨凌,712100
2. 中国农业科学院,畜牧研究所,北京,100094
摘    要:利用单链构向多态性方法检测了牛DGAT1基因第8外元AA→GC碱基突变,分析了该突变在三河牛、中国荷斯坦奶牛和中国西门塔尔奶牛中等位基因的分布频率,以及该突变对这3个品种奶牛群体产奶量、乳脂和乳蛋白含量的影响,并分析了该突变在鲁西牛、晋南牛、秦川牛和南阳牛中等位基因分布频率。结果表明,编码赖氨酸的等位基因K在三河牛、中国荷斯坦牛和中国西门塔尔牛中的分布频率分别为0.17,0.33和0.04,在鲁西牛、晋南牛、秦川牛和南阳牛4个中国地方品种中的分布频率分别为0.72,0.39,0.46和0.83;K232A取代与三河牛的平均乳脂率相关(P=0.017),KK和KA基因型个体的平均乳脂率分别较AA基因型个体高0.80%和0.41%。

关 键 词:DGAT1基因  奶牛  K232A取代
文章编号:1671-9387(2006)09-0006-05
收稿时间:2005-11-01
修稿时间:2005-11-01

Effects of the K232A substitution at DGAT1 gene on some economic traits in 3 chinese dairy cattle
XU Xiu-rong,GAO Xue,XU Shang-zhong,ZHANG Ying-han,LI Jun-ya,REN Hong-yan.Effects of the K232A substitution at DGAT1 gene on some economic traits in 3 chinese dairy cattle[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(9):6-10.
Authors:XU Xiu-rong  GAO Xue  XU Shang-zhong  ZHANG Ying-han  LI Jun-ya  REN Hong-yan
Institution:1 College of Animal Science,Northwest A & F University,Yangling,Shaanxi 712100,China; 2 Institute of Animal Science of the Chinese Academy of Agricultural Sciences,Beijing 100094,China)
Abstract:PCR-single-strand conformation polymorphism(PCR-SSCP) assay was used to diagnose the K232A substitution in the DGAT1 exon 8.Animals from three dairy cattle populations(212 Sanhe cattle,234 Chinese Holsteins and 200 Chinese Simmentals) were used to estimate allele frequencies and gene substitution effects on milk production traits.Another four populations(47 Luxi,23 Jinnan,37 Qinchuan and 39 Nanyang cattle) without production records were used to estimate allele frequencies only.The allele frequencies for the lysine-encoding variant(allele K) were 0.17,0.33 and 0.04 in Sanhe,Holstein,Simmental cows respectively;and 0.72,0.39,0.46 and 0.83 respectively in the other four populations.The average milk fat percentage in Sanhe cattle was associated(P=0.017) with the K232A substitution in DGAT1 gene,individuals with genotypes KK and KA had 0.80% and 0.41% higher average milk fat percentage than those with genotype AA.
Keywords:DGAT1 gene  dairy cattle  K232A substitution
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