| 猪繁殖与呼吸综合征病毒ORF7基因变异分析 |
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| 引用本文： | 曹素芳,王岩,肖松云,唐桂芬.猪繁殖与呼吸综合征病毒ORF7基因变异分析[J].郑州牧业工程高等专科学校学报,2009,29(1):1-3. |
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| 作者姓名： | 曹素芳 王岩 肖松云 唐桂芬 |
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| 作者单位： | 郑州牧业工程高等专科学校,河南,郑州,450011 |
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| 基金项目： | 河南省基础与前沿技术研究计划项目，河南省重点科技攻关项目，河南省高校科技创新人才支持计划 |
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| 摘 要： | 采用RT—PCR方法对疑似PRRS阳性病料进行克隆和测序获得了0RF7基因片段。序列分析结果表明获得的0RF7基因序列不存在缺失现象。与美洲型代表毒株VR-2332的核苷酸序列的同源性为91．3％，氨基酸同源性为92．6％；与我国最早的PRRSV分离株CH—la相比，核苷酸同源性为93．5％，氨基酸同源性为92．6％；与我国高致病性PRRSV毒株SD—ZQ的核苷酸同源性最高为98．1％，氨基酸只有两个不同，同源性为98．3％；与本省的Hn-1／06毒株的核苷酸同源性为97．3％，氨基酸同源性为97．5％；与2004年分离的FJ-2株与ORF7基因的核苷酸差异稍大为85．4％，氨基酸同源性为88．6％；与欧洲型分离株LV株和N-34株0RF7基因的核苷酸差异很大，同源性仅为41．1％和40％，氨基酸同源性为47．1％和47．6％。表明所获得序列的流行毒株属于美洲型，但其毒力已发生了变异。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF7基因 变异 |
Variation Analysis of Porcine Syndrome and Reproductive and Respiratory Virus ORF7 Gene |
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| CAO Su-fang,WANG Yan,XIAO Song-yun,TANG Gui-fen.Variation Analysis of Porcine Syndrome and Reproductive and Respiratory Virus ORF7 Gene[J].Journal of Zhengzhou College of Animal Husbandry Engineering,2009,29(1):1-3. |
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| Authors: | CAO Su-fang WANG Yan XIAO Song-yun TANG Gui-fen |
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| Institution: | Zhengzhou College of Animal Husbandry Engineering;Henan Zhengzhou 450011;China |
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| Abstract: | The ORF7 genes of pathologic material suspected PRRS was amplified by RT-PCR.The product was sequenced after cloning into pMD18-T vector.The sequence was analysed by DNAMAN software and compared with those of VR-2332,CH-la,SD-ZQ,Hn-1/06,FJ-2,Lv and N-34.it showed that the nucleotide sequence homology were 91.3%,93.5%,98.1%,97.3%,85.4%,41.1% and 40% respectivly.And the deduced amino acide homology were 92.6%,93.5%,98.3%,97.5%,88.6%,47.1%and 47.6%respectivly.It indicated that the isolate strain was related to... |
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| Keywords: | Porcine reproductive and respiratory syndrome vires ORF7 gene variation analysis |
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