Cholinergic activity of intestinal musclein vitro taken from horses with and without equine grass sickness |
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| Authors: | A Murray D F Cottrell M P Woodman |
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| Institution: | (1) Department of Preclinical Veterinary Sciences, Summerhall, EH9 1QH Edinburgh, UK;(2) Department of Veterinary Clinical Studies, Easter Bush, EH25 9RG Roslin, UK |
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| Abstract: | Equine grass sickness (EGS) is a pan-dysautonomia of horses that involves central and peripheral neuronal degeneration and ultimately depletion. This is the first reported functional study on the motility of equine intestine taken immediatelypost mortem from horses with EGS. Strips of smooth muscle from the small intestine of healthy and EGS-affected horses were suspended in an organ bath and their motility was measured isometrically. The activity of the cholinergic system was studied. Physostigmine enhanced the motility of all muscle strips. Tissues taken from horses suffering from acute grass sickness (AGS) had the longest latency before a measurable response could be obtained (p<0.05). The ileum appeared to be damaged by EGS to a greater extent than the duodenum. For the duodenal strips the enhanced rate of spontaneous contractions was significant (p<0.05) for both normal tissue and that affected by grass sickness but this was not the case for the ileal strips. Muscarinic receptor sensitivity investigation using bethanecol suggested a hypersensitivity of receptors with AGS material,Abbreviations AGS
acute grass sickness
- CGS
chronic grass sickness
- ED50
median effective dose
- EGS
equine grass sickness
- VIP
vasoactive intestinal peptide |
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| Keywords: | cholinergic dysautonomia grass sickness horse intestinal motility in vitro |
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