| 水稻OsWRKY17基因定位表达载体的构建(英文) |
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| 引用本文： | 王小兰,唐馨,刘忠渊.水稻OsWRKY17基因定位表达载体的构建(英文)[J].农业科学与技术,2012(1):79-81. |
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| 作者姓名： | 王小兰 唐馨 刘忠渊 |
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| 作者单位： | 广州大学生命科学学院;新疆大学生命科学与技术学院 |
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| 基金项目： | Supported by the National Natural Science Foundation of China(30971912);the Science and Technology Planning Project of the Colleges under Guangzhou Jurisdiction(08C030);the Guangzhou Science and Technology Planning Project(2008J1-C251-2);the"Yangcheng Scholars"Academic Backbone Project of the Colleges under Guangzhou Jurisdiction(10A042G)~~ |
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| 摘 要： | 目的]研究水稻OsWRKY17基因的生理生化特性,确定OsWRKY17蛋白在植物中的定位。方法]根据GenBank数据库中OsWRKY17全序列设计引物,进行OsWRKY17的RT-PCR扩增,克隆了OsWRKY17基因,将该片段与带绿色荧光蛋白(GFP)基因的质粒载体pBinGFP重组,将构建正确的表达载体pBinGFP-OsWRKY17通过农杆菌介导的花蕾浸泡法转化到拟南芥中。结果]经菌落PCR与酶切鉴定表明成功构建了Os-WRKY17基因与GFP融合的植物表达载体pBin-GFP/OsWRKY17,并成功将OsWRKY17基因整合到拟南芥的基因组中,获得了抗性植株。结论]OsWRKY17基因表达载体的构建为研究该基因的生理生化特性奠定了基础。
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| 关 键 词： | OsWRKY17基因 GFP基因 表达载体 |
Construction of OsWRKY17 Specific Expression Vector in Rice |
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| Xiaolan WANG,Xin TANG,Zhongyuan LIU.Construction of OsWRKY17 Specific Expression Vector in Rice[J].Agricultural Science ＆ Technology,2012(1):79-81. |
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| Authors: | Xiaolan WANG Xin TANG Zhongyuan LIU |
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| Institution: | 1.College of Life Science,Guangzhou University,Guangzhou 510006,China;2.College of Life Science and Technology,Xinjiang University,Urumqi 830046,China |
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| Abstract: | Objective] To study the physiological biochemical characteristic of OsWRKY17 in rice and identify the subcellular location of OsWRKY17.Method] The primer of the OsWRKY17 gene was designed according to the full-length sequence of OsWRKY17 in Genbank and was cloned by RT-PCR.The cloned fragment was then recombined with the green fluorescent protein gene of plasmid vector pBinGFP.The recombinant plasmid pBinGFP-OsWRKY17 was transformed into Arabidopsis through Agrobacterium tumefaciens strain GV3101.Result] Colony PCR and digestion identification proved that the plant expression vector pBinGFP-OsWRKY17 was successfully constructed by the fusion of OsWRKY17 and GFP,and the expression vector was successfully transformed into the genome of Arabidopsis,there by obtaining a resistant plant.Conclusion] The construction of OsWRKY17 expression vector established the foundation for study on the physiological the biochemical characteristics of OsWRKY17. |
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| Keywords: | OsWRKY17 Green fluorescent protein gene Expression vector |
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