| 小鼠卵巢HSP70诱导表达的免疫荧光组织化学研究 |
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| 引用本文： | 杜立银,田文儒.小鼠卵巢HSP70诱导表达的免疫荧光组织化学研究[J].黑龙江畜牧兽医,2006(11):22-24. |
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| 作者姓名： | 杜立银 田文儒 |
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| 作者单位： | 内蒙古民族大学 动物科技学院，莱阳农学院 动物科技学院 内蒙古 通辽028042，山东 青岛266109 |
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| 基金项目： | 国家自然科学基金项目(30270957)． |
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| 摘 要： | 试验采用免疫荧光组织化学法,检验了经历不同温度热应激后,在不同恢复期内小鼠卵巢组织HSP70的诱导表达,确立了HSP70诱导表达模式。结果表明：随着热应激温度的升高,卵巢组织HSP70的表达量增加,表达持续期延长,特别是经历了预应激小鼠的HSP70表达更显著。这说明在一定的热应激条件下。小鼠卵巢组织可发生HSP70的诱导表达,其表达量和表达持续期与热应激强度相关,而且热应激可显著促进HSP70的表达。
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| 关 键 词： | 昆明白鼠 热应激 卵巢 HSP70诱导表达 |
| 文章编号： | 1004-7034（2006）11-0022-03 |
| 收稿时间： | 2005-10-15 |
| 修稿时间： | 2005年10月15 |
Study on the induction of HSP70 expression in Ovary after Exposure of mouse to heat stress |
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| DU Li-yin,TIAN Wen-ru.Study on the induction of HSP70 expression in Ovary after Exposure of mouse to heat stress[J].Heilongjiang Animal Science And veterinary Medicine,2006(11):22-24. |
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| Authors: | DU Li-yin TIAN Wen-ru |
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| Institution: | 1. College of Animal Science and Teehnology of Inner Monglia University for Nationatities,Tongliao 028042, China; 2. College of Animal Science and Technology, Laiyang College ,Qingdao 265200, China |
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| Abstract: | Kunming(KM)mice were exposed to heat stress at different temperature respectively,in the purpose of revealiug and verifing the molecular nature of the ovary thermoprotective mechanisms and to extend the understanding for tissue speciality and immunohistochemistry method was performed to determine HSPTO induced expression of mouse ovarian section during the recovery after exposure of mice to heat stress.The results are as follows:Expression of HSP70 was induced in mouse ovary by heat stress.The quantity of expression of HSP70 increased and the duration was prolonged with the elevated temperature of heat stress,especially in the two-heat-stressed mice ovary.These results suggested that the quantity and duration of induced expression of HSP70 correlates with temperature of heat stress within certain range of heat stress,in particular,in the pre-heat-stressed mouse ovary. |
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| Keywords: | KM mouse heat stress ovary induction of HSP70 expression |
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