| 大肠杆菌毒力因子agn43与生物被膜表型相关性研究 |
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| 引用本文： | 陈朝喜,杨金福,陈伟明.大肠杆菌毒力因子agn43与生物被膜表型相关性研究[J].中国畜牧兽医,2012,39(4):76-78. |
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| 作者姓名： | 陈朝喜 杨金福 陈伟明 |
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| 作者单位： | 西南民族大学生命科学与技术学院,四川成都,610041 |
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| 基金项目： | 西南民族大学中央高校基本科研业务费专项资金项目(11NZYTD02),西南民族大学人才引进项目(2009RC005) |
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| 摘 要： | 分别采用改良结晶紫半定量方法和常规PCR方法对180株宠物源性大肠杆菌进行生物被膜形成能力定量研究和相变抗原agn43检测,以分析大肠杆菌毒力因子agn43与生物被膜表型相关性。试验结果表明,30.1%(55/180)的菌株能够检测到agn43基因,96.4%(53/55)agn43阳性菌株具有不同程度的成膜能力,与无成膜能力组差异极显著(P<0.01)。
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| 关 键 词： | 大肠杆菌 生物被膜 毒力因子 agn43 |
Studies on Correlation between E.coli Virulence Factor agn43 and Biofilm-forming Ability |
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| CHEN Chao-xi
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YANG Jin-fu
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CHEN Wei-ming.Studies on Correlation between E.coli Virulence Factor agn43 and Biofilm-forming Ability[J].China Animal Husbandry & Veterinary Medicine,2012,39(4):76-78. |
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| Authors: | CHEN Chao-xi YANG Jin-fu CHEN Wei-ming |
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| Institution: | College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China |
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| Abstract: | To elucidate the correlation between biofilm-forming ability and virulence factors,improved crystal violet semi-quantitative method and common PCR were used for biofilm-forming ability quantitative analysis and phase variation antigen 43 detection in 180 pet-brone E.coli respectively;the results revealed that 30.1%(55/180) of the strains detected agn43 and 96.4%(53/55)of the agn43 positive strains performed biofilm-forming ability,showing significant difference from non-biofilm forming group(P<0.01). |
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| Keywords: | E coli biofilm virulence factor agn43 |
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