Variation in Capsicum annuum revealed by RAPD and AFLP markers |
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| Authors: | Ilan Paran Ester Aftergoot Chen Shifriss |
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| Institution: | (1) Department of Genetics and Plant Breeding, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel |
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| Abstract: | Genetic relationships were examined among thirty-four pepper (Capsicum annuum) cultivars of different types. Two types of
PCR-based markers were used, RAPD and AFLP, and their relative effectiveness was compared. A dendrogram based on RAPD markers
separated the large-fruited sweet cultivars from the small-fruited pungent peppers, and the former group showed less divergence
than the latter. The percentage of polymorphic markers was lower for AFLP than for RAPD markers (13 and 22% respectively).
However, AFLP primers amplified on average six times more products than RAPD markers. The average numbers of polymorphic products
per primer were 1.6 and 6.5 for RAPD and AFLP primers, respectively, i.e., AFLP primers were four times more efficient than
RAPD primers in their ability to detect polymorphism in pepper. While four blocky type cultivars were indistinguishable by
RAPD, two AFLP primer pairs were sufficient to distinguish the four cultivars from each other.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | AFLP Capsicum annuum genetic distances RAPD |
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