| 血管内皮生长因子在绵羊肺脏中的表达分布研究 |
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| 引用本文： | 张忠华,谭紫仙,李静,杨琨.血管内皮生长因子在绵羊肺脏中的表达分布研究[J].畜牧与饲料科学,2019,40(6):13-15. |
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| 作者姓名： | 张忠华 谭紫仙 李静 杨琨 |
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| 作者单位： | 西北民族大学生命科学与工程学院,甘肃 兰州 730030 |
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| 摘 要： | 目的研究血管内皮生长因子（vascular endothelial growth factor,VEGF）在绵羊肺脏中的表达分布特征。方法取成年绵羊肺脏组织,制备石蜡切片,利用HE染色法观察绵羊肺脏组织的形态结构,采用免疫组织化学方法检测VEGF在绵羊肺脏组织中的分布。结果肺脏的各类型细胞均可见VEGF表达,在绵羊肺脏导气部的细支气管和终末细支气管的上皮细胞,呼吸部的肺泡管和呼吸性细支气管的上皮细胞,以及肺的血管内皮细胞均可检测到VEGF的强阳性表达信号。结论VEGF广泛分布于绵羊肺脏组织中,对其形态结构和功能的维持具有重要作用。
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| 关 键 词： | 血管内皮生长因子 绵羊 肺脏 免疫组织化学 |
| 收稿时间： | 2019-05-05 |
Expression and Distribution of Vascular Endothelial Growth Factor in Sheep Lung |
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| ZHANG Zhong-hua,TAN Zi-xian,LI Jing,YANG Kun.Expression and Distribution of Vascular Endothelial Growth Factor in Sheep Lung[J].Animal Husbandry and Feed Science,2019,40(6):13-15. |
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| Authors: | ZHANG Zhong-hua TAN Zi-xian LI Jing YANG Kun |
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| Institution: | College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China |
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| Abstract: | Objective] To characterize the expression and distribution of vascular endothelial growth factor （VEGF） in sheep lung. Method] The lung tissues of adult sheep were sampled to prepare the paraffin sections. The morphological structure of sheep lung tissues was observed by HE staining. The distribution of VEGF in sheep lung tissues was assessed by immunohistochemistry. Result] VEGF expression was observed in all types of lung cells. The strong positive expression signals of VEGF were detected in epithelial cells in bronchioles and terminal bronchioles of air conduction part of the lung, epithelial cells in alveolar ductules and respiratory bronchioles of the respiratory part of lung, and the vascular endothelial cells of the lung. Conclusion] VEGF was widely distributed in lung tissues of sheep and played an important role in the maintenance of lung morphological structure and function. |
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| Keywords: | VEGF sheep lung immunohistochemistry |
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