| 高效液相色谱法测定饲料中黄曲霉毒素含量的研究 |
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| 引用本文： | 赵贞,万鹏,李翠枝,吕志勇.高效液相色谱法测定饲料中黄曲霉毒素含量的研究[J].畜牧与饲料科学,2019,40(3):27-29. |
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| 作者姓名： | 赵贞 万鹏 李翠枝 吕志勇 |
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| 作者单位： | [1]内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特010010 |
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| 摘 要： | 旨在建立检测饲料中黄曲霉毒素B1、B2、G1、G2含量的高效液相色谱分析方法。样品用甲醇溶液提取,利用免疫亲和柱净化,以C18反相色谱柱分离,光化学衍生器衍生后应用荧光检测器检测。结果表明:4种黄曲霉毒素在0.5~20ng/mL范围内呈现良好的线性关系,回收率为71.6%~89.0%。采用该方法检测5种饲料样品中的黄曲霉毒素总量,检出率范围在74.0%~86.7%,检出值的范围为0.5~4.3μg/kg。建立的方法精密度高、重复性好,为监测饲料中黄曲霉毒素含量水平提供了有效的分析方法。
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| 关 键 词： | 饲料 高效液相色谱法 黄曲霉毒素 含量 |
| 收稿时间： | 2019-01-25 |
Determination of Aflatoxin in Feed by High-performance Liquid Chromatography |
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| ZHAO Zhen,WAN Peng,LI Cui-zhi,LU Zhi-yong.Determination of Aflatoxin in Feed by High-performance Liquid Chromatography[J].Animal Husbandry and Feed Science,2019,40(3):27-29. |
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| Authors: | ZHAO Zhen WAN Peng LI Cui-zhi LU Zhi-yong |
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| Institution: | [1]Inner Mongolia Yili Industrial Group Co.,Ltd.,Hohhot 010010,China |
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| Abstract: | The aim of this study was to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of aflatoxin B1,B2,G1 and G2 in feed.The sample was extracted with methanol solution,purified by immune-affinity column,separated by C18 reverse phase column,derivatized by photochemical derivatizer,and analyzed by fluorescence detector.The results showed that the four tested aflatoxins showed a good linear relationship in the range of 0.5-20 ng/mL,and the recovery was 71.6%-89.0%.The established HPLC method was used to detect the total amount of aflatoxin in five kinds of feed samples.The detection rate ranged from 74.0% to 86.7%,and the detected value of aflatoxin ranged from 0.5 to 4.3 μg/kg.In conclusion,the established HPLC method has high precision and good repeatability,providing an effective analytical method for monitoring the level of aflatoxin in feed. |
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