| 规模化驴场中马流感病毒H3N8亚型感染情况调查 |
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| 引用本文： | 于永峰,夏楠,孙晓波,庞雪晴,刘文强.规模化驴场中马流感病毒H3N8亚型感染情况调查[J].畜牧与饲料科学,2019,40(6):88-91. |
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| 作者姓名： | 于永峰 夏楠 孙晓波 庞雪晴 刘文强 |
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| 作者单位： | 聊城大学,山东 聊城 252000 |
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| 基金项目： | 山东省现代农业产业技术体系驴创新团队项目(SDAIT-27);国家级大学生科技创新创业训练计划(201810447011);聊城大学大学生创新创业训练项目(CXCY2018015) |
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| 摘 要： | 目的]调查山东省聊城市规模化驴场中马流感病毒的感染情况,并分析其可能的来源。方法]从聊城的规模化驴场采集病料和血清,通过HI试验检测驴血清中的马流感病毒H3N8亚型抗体的阳性率。使用RT-PCR技术扩增肺脏和鼻腔棉拭子样品中的马流感病毒M基因,对获得的马流感病毒M基因与不同流感病毒的M基因进行序列比对,推测其来源。结果]HI试验表明,120个血清样品中马流感病毒H3N8亚型血清抗体阳性率为33.3%(40/120);其中,母驴的马流感病毒H3N8亚型血清抗体阳性率为42.5%(17/40)、公驴为32.5%(13/40)、驴驹为25.0%(10/40)。通过RT-PCR检测发现,32.3%(21/65)的样品可测出目的条带。通过序列比对得出,该试验获得的流感病毒M基因与马属动物的H3N8亚型流感病毒高度同源(CY032222、CY032318、CY028821等),同源性最高可达99.8%。结论]马流感病毒在聊城周边的数个规模化养驴场发生流行。该研究从驴体内分离的流感病毒M基因属于马流感病毒H3N8亚型M基因。
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| 关 键 词： | 规模化驴场 马流感病毒 H3N8亚型 RT-PCR 抗体 |
| 收稿时间： | 2019-05-05 |
Prevalence of Equine Influenza Virus H3N8 Subtype Infection in Large-scale Donkey Farms |
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| YU Yong-feng,XIA Nan,SUN Xiao-bo,PANG Xue-qing,LIU Wen-qiang.Prevalence of Equine Influenza Virus H3N8 Subtype Infection in Large-scale Donkey Farms[J].Animal Husbandry and Feed Science,2019,40(6):88-91. |
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| Authors: | YU Yong-feng XIA Nan SUN Xiao-bo PANG Xue-qing LIU Wen-qiang |
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| Institution: | Liaocheng University,Liaocheng 252000,China |
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| Abstract: | Objective] To investigate the infection status of equine influenza virus in large-scale donkey farms in Liaocheng City of Shandong Province, and to identify the possible sources of the virus. Method] Clinical samples were collected from the large-scale donkey farms in Liaocheng City. The positive rate of serum antibodies against H3N8 subtype of equine influenza virus was determined by HI test. RT-PCR was used to amplify the M gene of equine influenza virus in lungs and nasal swabs. The obtained M gene was sequenced and compared with the M gene of different influenza viruses to infer its origin. Result] HI test showed that the positive rate of equine influenza virus H3N8 subtype in 120 serum samples was 33.3% （40/120）. The positive rate for female donkeys, male donkeys and donkey foals was 42.5% （17/40）, 32.5% （13/40） and 25.0% （10/40）, respectively. RT-PCR assay demonstrated that 32.3% （21/65） of the samples was detected as positive for the presence of M gene. The M gene obtained in this study is highly homologous to H3N8 subtype influenza virus of equine （CY032222, CY032318, CY028821, etc.）, with the highest homology of 99.8%. Conclusion] Equine influenza virus is prevalent in several large-scale donkey farms around Liaocheng City. The M gene of influenza virus obtained from donkeys in this study belongs to the M gene of H3N8 subtype of equine influenza virus. |
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| Keywords: | large-scale donkey farms equine influenza virus H3N8 subtype RT-PCR antibody |
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