| 20种中草药复配物对犬源葡萄球菌体外抑制作用的研究 |
| |
| 引用本文： | 聂佳伟,李淑红,彭华,郭泽婷.20种中草药复配物对犬源葡萄球菌体外抑制作用的研究[J].畜牧与饲料科学,2019,40(6):6-8. |
| |
| 作者姓名： | 聂佳伟 李淑红 彭华 郭泽婷 |
| |
| 作者单位： | 湖南文理学院生命与环境科学学院,湖南 常德 415000 |
| |
| 基金项目： | 湖南文理学院2018年大学生创新创业研究项目(ZC1830) |
| |
| 摘 要： | 为探究复方中草药对犬源葡萄球菌的体外抑制作用效果,将30种单味中草药配伍后得到20种复配物并制成水提物,采用二倍稀释法测定中草药复配物对犬源葡萄球菌的最低抑菌浓度(MIC),利用琼脂扩散法测定中草药复配物对犬源葡萄球菌的抑菌圈直径。结果表明,编号为15和16的中草药复配物对犬源葡萄球菌体外抑菌效果最好,最低抑菌浓度均为31.25mg/mL,抑菌圈直径分别为31.0mm和38.0mm,犬源葡萄球菌对这2个复方均表现为极敏;其他复方中草药对犬源葡萄球菌也呈现出不同程度的抑菌活性。研究结果为兽医临床筛选高效低毒的复方中草药制剂提供了科学依据。
|
| 关 键 词： | 复方中草药 犬源葡萄球菌 体外抑菌效果 最低抑菌浓度 抑菌圈直径 |
| 收稿时间： | 2019-04-26 |
Inhibitory Effect of 20 Kinds of Compound Chinese Herbal Medicine against a Clinical Staphylococcus Isolate from Canine in Vitro |
| |
| NIE Jia-wei,LI Shu-hong,PENG Hua,GUO Ze-ting.Inhibitory Effect of 20 Kinds of Compound Chinese Herbal Medicine against a Clinical Staphylococcus Isolate from Canine in Vitro[J].Animal Husbandry and Feed Science,2019,40(6):6-8. |
| |
| Authors: | NIE Jia-wei LI Shu-hong PENG Hua GUO Ze-ting |
| |
| Institution: | College of Life and Environmental Science，Hunan University of Arts and Sciences，Changde 415000，China |
| |
| Abstract: | This study aimed to explore the in vitro inhibitory effect of compound Chinese herbal medicine on a clinical Staphylococcus isolate from canine. The water extracts of a total of 20 kinds of compound Chinese herbal medicine were prepared with 30 kinds of single Chinese herbal medicine based on the traditional compatibility principle by using decoction method. The minimum inhibitory concentration （MIC） of the compound Chinese herbal medicine against the isolate was determined by using tube double dilution method, and the diameter of inhibition zone was determined by using agar diffusion method. The results showed that the NO. 15 and NO. 16 compound Chinese herbal medicine had the best antibacterial activity against the clinical isolate in vitro, and both of their MIC against the isolate was 31.25 mg/mL. The diameter of the inhibition zone of the NO. 15 and NO. 16 compound Chinese herbal medicine against the isolate was 31.0 mm and 38.0 mm, respectively, indicating that the isolate was extremely sensitive to both of the compounds. In addition, the other compound Chinese herbal medicine also showed certain antibacterial activity against the isolate. Our results provide a scientific basis for screening of high-efficient and low-toxic compound Chinese herbal preparation for veterinary clinical use. |
| |
| Keywords: | compound Chinese herbal medicine canine staphylococci antibacterial effect in vitro minimum inhibitory concentration inhibition zone diameter |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《畜牧与饲料科学》浏览原始摘要信息 |
| 点击此处可从《畜牧与饲料科学》下载免费的PDF全文 |
