| 外源褪黑激素对罕山白绒山羊绒毛生长相关基因表达差异的影响 |
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| 引用本文： | 丽春,李金泉,张文广,王思珍,王国富,高树新,付绍印.外源褪黑激素对罕山白绒山羊绒毛生长相关基因表达差异的影响[J].畜牧与饲料科学,2019,40(9):11-16. |
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| 作者姓名： | 丽春 李金泉 张文广 王思珍 王国富 高树新 付绍印 |
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| 作者单位： | 1.内蒙古民族大学动物科学技术学院,内蒙古 通辽 028000;2.内蒙古农业大学动物科学学院,内蒙古 呼和浩特 010018;3.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031;4.内蒙古ATCG生物信息研究所,内蒙古 呼和浩特 010020 |
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| 摘 要： | 试验旨在筛选罕山白绒山羊皮肤毛囊生长脱落相关的关键基因,并揭示外源褪黑激素（Melatonin,MT）对罕山白绒山羊绒毛生长相关基因表达差异的影响。选择10只体重平均为33.3 kg、年龄为20月龄的罕山白绒山为实验动物,分为2组,从2014年12月开始每隔1个月按照2 mg/（kg·BW）的剂量在埋植组绒山羊耳后皮下埋植MT,于2015年9月采集2组罕山白绒山羊皮肤组织进行RNA-Seq测序,对差异表达基因进行筛选、功能注释和富集分析。共筛选获得了475个在埋植组与对照组中差异表达的基因,其中,上调表达基因392个,下调表达基因83个。KEGG富集性分析表明,差异表达基因主要参与白细胞经内皮迁移、趋化因子信号通路、fcγ-R介导的吞噬作用、吞噬体、造血细胞谱系、补体级联和混凝级联、细胞因子—细胞因子受体相互作用、NF-kappa B信号通路、PI3K-Akt信号通路等。PI3K-Akt信号通路可能在褪黑激素调控皮肤毛囊发育中发挥重要作用。研究结果为进一步研究MT影响皮肤毛囊生长的分子机理提供依据。
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| 关 键 词： | 褪黑激素 罕山白绒山羊 差异表达基因 |
| 收稿时间： | 2019-09-06 |
Effects of Exogenous Melatonin on Expressional Differences of Cashmere Growth Related Genes in Hanshan White Cashmere Goats |
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| Lichun,LI Jin-quan,ZHANG Wen-guang,WANG Si-zhen,WANG Guo-fu,GAO Shu-xin,FU Shao-yin.Effects of Exogenous Melatonin on Expressional Differences of Cashmere Growth Related Genes in Hanshan White Cashmere Goats[J].Animal Husbandry and Feed Science,2019,40(9):11-16. |
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| Authors: | Lichun LI Jin-quan ZHANG Wen-guang WANG Si-zhen WANG Guo-fu GAO Shu-xin FU Shao-yin |
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| Institution: | 1.College of Animal Science and Technology,Inner Mongolia University for Nationalities,Tongliao 028000,China;2.College of Animal Sciences,Inner Mongolia Agricultural University,Hohhot 010018,China;3.Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;4.Inner Mongolia ATCG Bioinformation Institute,Hohhot 010020,China |
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| Abstract: | The aims of this study were to screen the key genes associated with growth and loss of cashmere in skin follicle in Hanshan white cashmere goats, and to reveal the effects of exogenous melatonin （MT） on the expression of genes related to cashmere growth in Hanshan white cashmere goats. A total of 10 Hanshan white cashmere goats aged 20 months with average body weight of 33.3 kg were selected as experimental animals and were divided into two groups. The goats in the experimental group received a subcutaneous implantation of MT in skin behind ear at a dose of 2 mg/（kg·BW） every 1 month from December 2014 to December 2015. The skin tissues of the goats in the two groups were collected in September 2015 for RNA-Seq sequencing, and the screened differential expressed genes were subjected to function annotation and enrichment analysis. A total of 475 differentially expressed genes were screened between experimental group and control group, of which 392 were up-regulated and 83 were down-regulated. KEGG enrichment analysis showed that the differentially expressed genes were mainly involved in leukocyte endothelial migration, chemokine signaling pathway, fcγ-R-mediated phagocytosis, phage, hematopoietic lineage, complement and coagulation cascades, cytokine-cytokine receptor interaction, NF-kappa B signaling pathway, PI3K-Akt signaling pathway, etc. PI3K-Akt signaling pathway may play an important role in MT-regulated skin follicle development. Our results provide a basis for further study on the molecular regulative mechanism of MT in skin follicle growth. |
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| Keywords: | melatonin Hanshan white cashmere goats differentially expressed genes |
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