| 生物反应器在金线莲扩繁中的应用研究 |
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| 引用本文： | 吴荣哲.生物反应器在金线莲扩繁中的应用研究[J].安徽农业科学,2010,38(36):20571-20573,20591. |
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| 作者姓名： | 吴荣哲 |
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| 作者单位： | 延边大学农学院园艺系,吉林延吉,133002 |
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| 摘 要： | 目的]研究在自动化、低成本的生物反应器内大量扩繁金线莲。方法]把金线莲丛状芽培养在不同环境条件下的3 L球形生物反应器中,通过加入花宝(20-20-20)1 g/L+花宝(6.5-4.5-19.0)1 g/L培养基,同时添加蛋白胨2.0 g/L、蔗糖3%和颗粒活性炭0.5 g/L,比较了凝胶培养基与不同条件下生物反应器(浸没式、接触式和潮汐式)培养的金线莲丛生芽的生长情况。结果]植株生长(干重、茎高、叶数、根数和根长)在接触式生物反应器中相对较好。当外植体数目在60～90个、通气量在0.06 vvm时测试结果(鲜重、茎长、叶数、叶面积和根数)最好,再生苗拥有良好的根系且在温室易驯化成功。结论]利用生物反应器扩繁金线莲是一种新的有效途径。
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| 关 键 词： | 金线莲 球形生物反应器 扩繁 潮汐式 |
Application of Bioreactor System in Multiplication of Anoectochilus formosanus |
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| WU Rong-zhe.Application of Bioreactor System in Multiplication of Anoectochilus formosanus[J].Journal of Anhui Agricultural Sciences,2010,38(36):20571-20573,20591. |
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| Authors: | WU Rong-zhe |
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| Institution: | WU Rong-zhe (Department of Horticulture; Agricultural College of Yanbian University; Yanji; Jilin 133002); |
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| Abstract: | Objective] The aim was to research the application of automated,low cost bioreactor system in in vitro mass propagation of Anoectochilus formosanus.Method] The shoot tips of Anoectochilus formosanus were cultured in 3 L balloon type bubble bioreactor(BTBB) containing the medium with 1 g/L Hyponex(20-20-20),1g/L Hyponex(6.5-4.5-19.0),2.0 g/L peptone,3% sucrose and 0.5 g/L granular activated charcoal under different culture conditions(continuous immersion with or without net and temporary immersion in liquid media using ebb and flood).Result] The plant growth(measured in terms of dry weight,shoot length,number of leaves,root number and length) was higher in continuous immersion(with net) bioreactor culture.Under the conditions of 60-90 explants per bioreactor,0.06 vvm of ventilatory capacity,the comprehensive results of fresh weight,shoot length,leaf number,leaf area and root number was the best,and the regenerated seedling with well developed roots were successfully acclimatized and transferred to greenhouse.Conclusion] The application of bioreactor was a new method for propagation of A.formosanus plantlets. |
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| Keywords: | Anoectochilus formosanus Balloon type bubble bioreactor Micropropagation Temporary immersion |
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