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| 鸽热休克蛋白70基因的扩增与同源性比较 | |
| 引用本文： | 薛茂云,赵宝华,吕玲,刘萍,高琴,戴鼎震.鸽热休克蛋白70基因的扩增与同源性比较[J].安徽农业科学,2010,38(36). |
| 作者姓名： | 薛茂云 赵宝华 吕玲 刘萍 高琴 戴鼎震 |
| 作者单位： | 1. 江苏经贸职业技术学院,江苏南京,211196 2. 中国农业科学院家禽研究所,江苏扬州,225700 3. 金陵科技学院,江苏南京,211196 |
| 基金项目： | 江苏省高校科研成果产业化推进项目 |
| 摘 要： | 目的]扩增鸽热休克蛋白(HSP)70基因,并与其他禽类作同源性比较。方法]参考禽类的HSP70基因序列设计、合成引物,从热应激鸽肝脏组织中提取总RNA作为模板,RT-PCR扩增目的基因。结果]RT-PCR扩增到的基因片段为576 bp,经测序,确定为鸽HSP70基因;鸽HSP70基因与鸡、鹌鹑、珍珠鸡同类基因序列的同源性达97%以上。结论]禽类的HSP70基因具有较高保守性。 |
| 关 键 词： | 肉鸽 热休克蛋白70 RT-PCR 同源性 |
Amplifying and Homology Comparison of Heat Shock Protein 70 Gene in Pigeon |
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| Institution: | XUE Mao-yun et al (Jiangsu Institute of Economic and Trade Technology; Nanjing; Jiangsu 211196); |
| Abstract: | Objective] To amplify and do homology comparison of heat shock protein(HSP)70 gene in pigeon.Method] A pair of primers were designed and synthesized using HSP70 gene sequence in poultry as the reference sequence.Total RNA was extracted from livers of heat-stressed pigeons,and the objective gene was amplified by RT-PCR.Result] The gene fragments amplified by RT-PCR was about 576 bp,and was confirmed as HSP70 gene in pigeon by sequence measurement.The homology of HSP70 gene in pigeon was over 97%,compared with chicken,quail and guinea fowl.Conclusion]HSP70 gene in poultry has high conservation. |
| Keywords: | RT-PCR |
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