| RAPD法研究孝感荸荠和野生荸荠的遗传差异性 |
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| 引用本文： | 曹姣,李建华,盛继群.RAPD法研究孝感荸荠和野生荸荠的遗传差异性[J].安徽农业科学,2010,38(36):20551-20553. |
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| 作者姓名： | 曹姣 李建华 盛继群 |
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| 作者单位： | 孝感学院生命科学技术学院,湖北省作物病害监测和安全控制重点实验室,湖北孝感,432000;孝感学院生命科学技术学院,湖北省作物病害监测和安全控制重点实验室,湖北孝感,432000;孝感学院生命科学技术学院,湖北省作物病害监测和安全控制重点实验室,湖北孝感,432000 |
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| 基金项目： | 湖北省自然科学基金项目，湖北省教育厅重大项目 |
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| 摘 要： | 目的]采用RAPD法研究孝感荸荠和野生荸荠的遗传差异性。方法]利用随机扩增多态性DNA(RAPD)技术,对地方栽培种孝感荸荠、野生荸荠、蒲草和扁杆藨草的基因组DNA进行了遗传差异性分析。结果]共筛选出841、842、807和840 4个随机引物,其中841随机引物的扩增产物多态性明显,获得清晰、重复性好的条带。聚类分析结果显示,栽培荸荠与野生荸荠的亲缘性相对于蒲草与扁杆藨草而言较近。结论]为培植优质荸荠新品种提供了理论依据。
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| 关 键 词： | 荸荠 RAPD 聚类分析 遗传差异性 |
Genetic Diversities between Xiaogan Water Chestnut and Wild Chestnut with Randomly Amplified Polymorphic DNA Technology |
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| Institution: | CAO Jiao et al(Key Lab of Crop Disease Monitoring & Safety Control in Hubei Province of School of Life Science and Technology of Xiaogan University; Xiaogan; Hubei 432000); |
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| Abstract: | Objective] The aim was to study the genetic diversities between Xiaogan water chestnut and wild chestnut with randomly amplified polymorphic DNA(RAPD) technology.Method] Genetic diversities of the local cultivated water chestnut,wild chestnut,Lepironia articulate and Scirpus planiculmis Fr.Schmidt were analyzed by RAPD technology.Result] Among the screened random primers 841,842,807 and 840,the polymorphism of amplification product of 841 was evident,and the obtained bands in electrophoresis were clear and showed good repeatability.Cluster analysis result showed that the affinity of cultivated water chestnut and wild water chestnut was nearer than that between Lepironia articulate and Scirpus planiculmis Fr.Schmidt.Conclusion] The research provides theoretical basis for cultivating high-quality new varieties of water chestnut. |
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| Keywords: | RAPD |
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