| 伏毛铁棒锤提取物对菜青虫的毒杀和拒食活性研究 |
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| 引用本文： | 梁永锋,刘立红,刘世巍,丁建海.伏毛铁棒锤提取物对菜青虫的毒杀和拒食活性研究[J].安徽农业科学,2010,38(36):20649-20650,20652. |
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| 作者姓名： | 梁永锋 刘立红 刘世巍 丁建海 |
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| 作者单位： | 宁夏师范学院化学化工学院,宁夏固原,756000;宁夏师范学院化学化工学院,宁夏固原,756000;宁夏师范学院化学化工学院,宁夏固原,756000;宁夏师范学院化学化工学院,宁夏固原,756000 |
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| 基金项目： | 宁夏回族自治区科技攻关项目资助 |
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| 摘 要： | 目的]研究伏毛铁棒锤(Aconitumflavum)对菜青虫(Pieris rapae)的毒杀和拒食活性。方法]采用浸虫法测定伏毛铁棒锤乙醇粗提物和石油醚、乙醚、乙酸乙酯、正丁醇和水5种不同极性溶剂萃取物对菜青虫的触杀活性;采用叶蝶法测定石油醚、乙醚、正丁醇、乙酸乙酯、水的5种溶剂萃取物对菜青虫的拒食活性。结果]伏毛铁棒锤粗提物对菜青虫具有较高的触杀活性,浓度为100.00 mg/ml时,48h的校正死亡率达97.24%,5种萃取物对菜青虫的触杀活性次序为水>正丁醇>乙酸乙酯>乙醚>石油醚,水萃取物48 h的校正死亡率达95.87%;5种溶剂萃取物拒食活性顺序为水>正丁醇>乙酸乙酯>乙醚>石油醚。结论]伏毛铁棒锤提取物对菜青虫具有较强的触杀和拒食活性,且杀虫和拒食活性物质是一类极性较大的化合物。
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| 关 键 词： | 伏毛铁棒锤 提取物 菜青虫 毒杀 拒食活性 |
Contact Toxicity and Anti-feeding Activity of Aconitum flavum Extract against Cabbage Worm |
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| Institution: | LIANG Yong-feng et al(College of Chemistry and Chemical Engineering; Ningxia Normal University; Guyuan; Ningxia 756000); |
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| Abstract: | Objective]The paper was to study the contact toxicity and anti-feeding activity of Aconitum flavum against cabbage worm.Method]Insect dipping method was adopted to determine the contact toxicity of the extracts of A.flavum extracted from five polar solvents including ethanol,petroleum ether,ether,ethyl acetate,n-butanol and water;leaf dish method was adopted to determine the anti-feeding activities of five solvent extracts including ethanol,petroleum ether,ether,ethyl acetate,n-butanol and water against cabbage worm.Result]Extracts of A.flavum had high contact toxicity against cabbage worm,when the concentration was 100.00 mg/ml,the corrected mortality at 48 h reached 97.24%,and the insecticidal activities of five solvent extracts against cabbage worm in sequence were water>n-butanol>ethyl acetate>ether>petroleum ether,the corrected mortality of water extract at 48 h was 95.87%;the anti-feeding activities of five solvent extracts in sequence were water>n-butanol>ethyl acetate>ether>petroleum ether.Conclusion]Extracts of A.flavum had strong contact toxicity and anti-feeding activity against cabbage worm,and the active ingredients with contact toxicity and anti-feeding activity might be a kind of polar compound. |
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| Keywords: | A flavum Extract Cabbage worm Contact toxicity Anti-feeding activity |
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