| 棉花转基因材料的获得及主要农艺性状变异分析 |
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| 引用本文： | 刘方,王坤波,宋国立,黎绍惠,王春英,张香娣,王玉红,胡育昌.棉花转基因材料的获得及主要农艺性状变异分析[J].棉花学报,2009,21(1):23-27. |
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| 作者姓名： | 刘方 王坤波 宋国立 黎绍惠 王春英 张香娣 王玉红 胡育昌 |
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| 作者单位： | 中国农业科学院棉花研究所,农业部棉花遗传改良重点开放实验室,河南,安阳,455000 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，国家科技支撑计划 |
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| 摘 要： | 利用不同棉花受体材料进行花粉管通道法遗传转化研究。通过卡那霉素鉴定、抗虫性鉴定和PCR分子生物学鉴定，证明已成功将Bt基因转化到泗棉3号、辽棉15、中棉所19、中棉所29母本、中棉所35、中棉所36等棉花材料中。通过对转基因材料后代与非转基因材料的铃重、衣分和纤维品质等差异分析，发现花粉管通道转基因后代材料存在广泛的变异。
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| 关 键 词： | 棉花 花粉管通道 转基因 遗传变异 |
Variations of Main Agronomic Traits in Transgenic Cotton (Gossypium hirsutum L.) Lines |
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| LIU Fang,WANG Kun-bo,SONG Guo-li,LI Shao-hui,WANG Chun-ying,ZHANG Xiang-di,WANG Yu-hong,HU Yu-chang.Variations of Main Agronomic Traits in Transgenic Cotton (Gossypium hirsutum L.) Lines[J].Cotton Science,2009,21(1):23-27. |
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| Authors: | LIU Fang WANG Kun-bo SONG Guo-li LI Shao-hui WANG Chun-ying ZHANG Xiang-di WANG Yu-hong HU Yu-chang |
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| Institution: | Cotton Research Institute, CAAS, Key Laboratory of Cotton Genetic Improvement, MOA, Anyang, Henan 455000, China |
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| Abstract: | Different cotton lines have been studied in inheritance traits changing by pollen transgenic method. Bt gene has been successfully transformed into Simian 3, Liaomian 15, CCRI 19, CCRI 29, CCRI 35 and CCRI 36 cotton varieties by pollen transgenic method which was correspondingly confirmed by kanamysin judging, insect-resistance detecting, PCR verifying and southern bloting. The SAS analyses indicated that pollen transgenic cotton had large changing in inheritance characteristics with the boll weight, ginning outturn and fiber qualities. |
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| Keywords: | pollen-tube pathway transgenic genetic variation |
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