| 转基因植物中T-DNA整合的分子特征及表达 |
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| 引用本文： | 杨晓杰,刘传亮,张雪妍,李付广.转基因植物中T-DNA整合的分子特征及表达[J].广西农业生物科学,2010(1):125-130. |
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| 作者姓名： | 杨晓杰 刘传亮 张雪妍 李付广 |
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| 作者单位： | 中国农业科学院棉花研究所;农业部棉花遗传改良重点开放实验室; |
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| 基金项目： | 国家863项目(2006AA100105)资助 |
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| 摘 要： | 植物中不同转基因方法转化外源基因的T-DNA整合特征既具有共性,又具有特性,使得转基因的遗传在各独立转化体间呈现多样性,另外多种遗传因子和限制因素使受体植物中外源基因的表达存在下降,甚至出现基因沉默等复杂现象。本文主要对农杆菌介导及裸露DNA直接转化转基因植物中T-DNA的分子特征和转基因表达的影响因子进行了介绍和概述。转化体中转基因的遗传稳定性和表达主要取决于转基因在植物基因组中的整合位置、拷贝数及组成结构。因而,通过对具有表达水平各异的转化体进行深入的遗传分析和分子生物学研究以及转化体之间进行的比较研究,将对转基因技术自身的完善、定点整合以及更有效的利用转基因技术都具有十分重要的意义。
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| 关 键 词： | 转基因植物 T-DNA整合 转基因遗传和表达 |
Molecule Characterization and Expression of T-DNA Integration in Transformed Plants |
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| Yang Xiaojie Liu Chuanliang Zhang Xueyan Li Fuguang Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang.Molecule Characterization and Expression of T-DNA Integration in Transformed Plants[J].Journal of Guangxi Agricultural and Biological Science,2010(1):125-130. |
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| Authors: | Yang Xiaojie Liu Chuanliang Zhang Xueyan Li Fuguang Key Laboratory of Cotton Genetic Improvement Ministry of Agriculture Cotton Research Institute Chinese Academy of Agricultural Sciences Anyang |
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| Institution: | Yang Xiaojie Liu Chuanliang Zhang Xueyan Li Fuguang Key Laboratory of Cotton Genetic Improvement,Ministry of Agriculture,Cotton Research Institute,Chinese Academy of Agricultural Sciences,Anyang,455000 |
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| Abstract: | The characterization of T-DNA integration of different transgenic methods transfering genes in plants has common characteristics,as well as specific characteristics,which makes the hereditary of transgene presenting diversity in different independence transformed plants. In addition,the expression of foreign genes in receptor plants represents down-regulated,even some complicated phenomenon are found,such as gene silencing un-der multi-genetic factors and limitation factor. In this article,we chiefly introd... |
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| Keywords: | Transgenic plant T-DNA integration Transgene inheritence and expression |
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