| 云杉叶疫病根球壳孢菌的分子检测 |
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| 引用本文： | 许秀兰,张岩,杨春琳,袁川,刘应高.云杉叶疫病根球壳孢菌的分子检测[J].湖南农业大学学报(自然科学版),2016,42(1):70-75. |
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| 作者姓名： | 许秀兰 张岩 杨春琳 袁川 刘应高 |
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| 作者单位： | 1. 四川农业大学林学院,四川成都,611130;2. 西藏林业调查规划研究院,西藏拉萨,850032;3. 四川省广安市林业局,四川广安,638550 |
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| 摘 要： | 从四川二郎山人工云杉林分离得到云杉叶疫病病原菌,采用真菌通用引物ITS1和ITS4扩增得到的序列与NCBI中的根球壳孢菌(Rhizosphaera kalkhoffi)相似度达99%。比较分析了NCBI及四川二郎山分离到的根球壳孢菌ITS区序列,采用软件primer5.0设计20个引物。通过对32株菌株DNA进行PCR扩增及产物测序,筛选得到3个特异性引物对ZYZ6、ZYZ7、ZYZ8。改变DNA模板浓度进行扩增检测,在25μL PCR反应体系中,引物对ZYZ6F/ZYZ6R检测灵敏度最高,可达到0.1 pg/μL。利用ZYZ6F/ZYZ6R对四川二郎山人工云杉针叶DNA进行检测,扩增得到310 bp左右的条带,测序结果与Gen Bank中根球壳孢菌相似度达99%。
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| 关 键 词： | 云杉叶疫病 根球壳孢菌 分子检测 |
The Determining Factors of Payment of M&A of ST Listed Company from Two Positions: An Empirical Study on Chinese Capital Market Based on the Logistic Model |
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| ZHENG Zhu-qing,XIE Chi.The Determining Factors of Payment of M&A of ST Listed Company from Two Positions: An Empirical Study on Chinese Capital Market Based on the Logistic Model[J].Journal of Hunan Agricultural University,2016,42(1):70-75. |
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| Authors: | ZHENG Zhu-qing XIE Chi |
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| Institution: | (1. Business School, Hunan University, Changsha, Hunan410082, China; 2. Research Center for Finance and Investment, Hunan University, Changsha, Hunan410082, China) |
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| Abstract: | This paper uses the Logistic model to study the payment of M&A of ST listed company as either the bidder side or the target side. We study the determining factors of payment of M&A of ST listed Company from three aspects: market factors, corporate factors and transaction factors. We find that, where there is different of merger position of ST listed companies, there is different of payment of M&A. Furthermore, when the ST listed company acts as the bidder in M&A, the payment should be influenced by the regulator right, number of analysts and hostile deals. However, when the ST listed company acts as the target in M&A, the payment should be governed by B/M, leverage, number of analysts and hostile deals. In brief, the difference of payment of M&A has an effect on different performances, and therefore analyzing the relevant determinants of ST listed companies that choosing more appropriate payment of M&A contributes to achieve the expected value after the merger. |
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| Keywords: | spruce needle blight Rhizosphaera kalkhoffii molecular detection |
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