| 水稻根际固氮菌NX-2的分离鉴定及其培养条件优化 |
| |
| 引用本文： | 杜宇,李来梅,雷潇,杜东霞,尹红梅,刘标,许隽.水稻根际固氮菌NX-2的分离鉴定及其培养条件优化[J].湖南农业科学,2017(7). |
| |
| 作者姓名： | 杜宇 李来梅 雷潇 杜东霞 尹红梅 刘标 许隽 |
| |
| 作者单位： | 湖南省微生物研究院,湖南长沙,410009 |
| |
| 摘 要： | 筛选高效固氮能力的植物根际促生菌是研发微生物肥料的关键。研究从水稻根际土壤中分离筛选得到一株固氮菌NX-2,利用乙炔还原法测定其固氮酶活可达16.18μmol/(m L·h)(以C2H4浓度计)。通过形态观察、生理生化特性和16S r DNA序列分析,菌株NX-2被鉴定为褐球固氮菌。进一步研究其生长特性,结果显示最佳培养条件为培养温度28℃、初始p H值6.5、接种量3%。
|
| 关 键 词： | 水稻根际 固氮微生物 分离 鉴定 培养条件优化 |
Isolation,Identification and Optimization of culture conditions of Nitrogen-Fixing Bacteria NX-2 from Rice Rhizosphere |
| |
| DU Yu,LI Lai-mei,LEI Xiao,DU Dong-xia,YIN Hong-mei,LIU Biao,XU Jun.Isolation,Identification and Optimization of culture conditions of Nitrogen-Fixing Bacteria NX-2 from Rice Rhizosphere[J].Hunan Agricultural Sciences,2017(7). |
| |
| Authors: | DU Yu LI Lai-mei LEI Xiao DU Dong-xia YIN Hong-mei LIU Biao XU Jun |
| |
| Abstract: | It is a key issue to screen the rhizosphere promoting bacteria of high efficient nitrogen fixing ability for the development of microbial fertilizer. In this study a nitrogen-fixing bacteria NX-2 was isolated from rice rhizosphere soil. Nitrogenase activity was measured by acetylene reduction at 16.18 μmol/(mL·h) calculated C2H4concentration. Through morphological observation, physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain NX-2 was identified as Azotobacter. Its growth characteristics were further studied, the results showed that the optimal fermentation condition was the initial temperature 28℃, pH 6.5, inoculum size 3%. |
| |
| Keywords: | rice rhizosphere nitrogen-fixing bacteria isolation identification optimization of culture conditions |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
