| 猪繁殖与呼吸综合征病毒GP5基因及猪IL-18基因共表达真核表达系统的建立 |
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| 引用本文： | 李国江,冯立文,高磊,丁壮,张立春.猪繁殖与呼吸综合征病毒GP5基因及猪IL-18基因共表达真核表达系统的建立[J].中国兽医学报,2011,31(9). |
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| 作者姓名： | 李国江 冯立文 高磊 丁壮 张立春 |
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| 作者单位： | 吉林农业科技学院,吉林吉林,132101 |
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| 基金项目： | 广东省科技攻关重大项目(2008A020100020) |
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| 摘 要： | 采用RT-PCR方法扩增了JL/07/SW毒株GP5蛋白和猪IL-18基因。将该基因克隆入真核表达载体pEG-FP-N1,获得重组质粒pEGFP-GP5和pEGFP-IL18-GP5。将重组质粒转染Marc-145细胞,通过Western blotting和green fluorescent检测产物的表达情况。结果显示,所构建的2个重组质粒在Marc-145细胞中能进行有效的转录。结果表明,所构建的重组质粒pEGFP-GP5和pEGFP-IL18-GP5在Marc-145细胞中得到表达,为进一步研究PRRSV基因工程疫苗奠定了基础。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 GP5 IL-18 核酸疫苗 |
Construction and identification of DNA vaccine co-expressing swine IL-18 and GP5 protein of porcine reproductive and respiratory syndrome virus |
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| LI Guo-jiang,FENG Li-wen,GAO Lei,DING Zhuang,ZHANG Li-chun.Construction and identification of DNA vaccine co-expressing swine IL-18 and GP5 protein of porcine reproductive and respiratory syndrome virus[J].Chinese Journal of Veterinary Science,2011,31(9). |
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| Authors: | LI Guo-jiang FENG Li-wen GAO Lei DING Zhuang ZHANG Li-chun |
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| Institution: | LI Guo-jiang1,2,FENG Li-wen1,GAO Lei3,DING Zhuang1,ZHANG Li-chun2 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China,2.College of Animal Veterinary Medicine,College of Jilin Agricultural Science and Technology,Jilin City,Jilin 132101,3.College of Animal Science and Technology,Jilin Agricultural University,China) |
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| Abstract: | Swine IL-18 and GP5 protein of the JL/07/SW strain of PRRSV was amplified using RT-PCR,and then cloned into eukaryotic expression vector pEGFP-N1 to construct recombinant plasmid pEGFP-GP5 and pEGFP-IL18-GP5,and then transfer to Marc-145 cells.Expression products were detected by Western blot-ting and green fluorescent.These two recombinant plasmid pEGFP-GP5 and pEGFP-IL18-GP5 were transcribed effectively and the objective proteins were expressed in Marc-145 cells.The recombinant expression vector pEGFP-GP5... |
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| Keywords: | porcine reproductive and respiratory syndrome virus(PRRSV) GP5 IL-18 DNA vaccines |
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