| 悬浮培养生产口蹄疫病毒灭活工艺研究 |
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| 引用本文： | 乔亮明.悬浮培养生产口蹄疫病毒灭活工艺研究[J].中国兽药杂志,2012,46(5):37-39. |
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| 作者姓名： | 乔亮明 |
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| 作者单位： | 金宇保灵生物药品有限公司,呼和浩特,010030 |
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| 摘 要： | 采用单独使用二乙烯亚胺（BEI）灭活剂30℃、26℃灭活以及二乙烯亚胺-甲醛（BEI-FA）联合灭活3种方法对悬浮培养生产的猪口蹄疫O型ZK/93病毒液进行灭活,考察和评价各种方法对该毒株146S的影响及灭活安全性。根据对3批口蹄疫病毒灭活后检测结果来看,单独使用BEI 26℃灭活方法虽然对146S的损失率小,但存在一定的安全隐患,未经过大量试验证实及确认之前不应使用。而经BEI-FA联合灭活方法获得的抗原安全性检验符合规定,但该方法对口蹄疫抗原146S的损失率较高,不宜推广和使用。与上述两种方法相比,用BEI灭活剂30℃灭活28 h仍是最佳的灭活方法。
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| 关 键 词： | 悬浮培养 口蹄疫病毒 灭活工艺 |
| 收稿时间： | 2011/11/17 0:00:00 |
| 修稿时间： | 2012/3/13 0:00:00 |
Study on Inactivation Technology of Foot and Mouth Disease Virus Produced by Suspension Culture |
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| qiaoliangming.Study on Inactivation Technology of Foot and Mouth Disease Virus Produced by Suspension Culture[J].Chinese Journal of Veterinary Drug,2012,46(5):37-39. |
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| Authors: | qiaoliangming |
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| Institution: | jinyubaolingshengwuyaopinyouxiangongsi |
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| Abstract: | In order to investigate and evaluate the effect on 146S and the security of inactivation,three different inactivation methods were respectively used to inactivate the O type ZK/93 strain of foot and mouth disease virus produced by suspension culture.These three inactivation methods were BEI inactivation alone at 30 ℃,BEI inactivation alone at 26 ℃ and BEI-FA combined inactivation.From the test result of three batches of inactivation,we can conclude that the loss rate of 146S was small using BEI alone at 26 ℃,but the security risk existed.This method should not be used before it is proved and confirmed by a lot of tests.The method of combined BEI-FA had high security,but the loss rate of 146S was high and should not be promoted and used.Comparing with two methods above,BEI alone at 30 ℃ for 28 hours is still the best method. |
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| Keywords: | suspension culture foot and mouth disease inactivation technology |
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