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| 兽医临床凝固酶阴性葡萄球菌分离株的耐药性及耐药基因检测 | |
| 引用本文： | 张纯萍,陈惠娟,宋立,宁宜宝,马丽.兽医临床凝固酶阴性葡萄球菌分离株的耐药性及耐药基因检测[J].中国兽医学报,2011,31(11). |
| 作者姓名： | 张纯萍 陈惠娟 宋立 宁宜宝 马丽 |
| 作者单位： | 1. 中国兽医药品监察所,北京,100081 2. 扬州大学江苏扬州225009 3. 洛阳惠中兽药有限公司,河南洛阳,471003 |
| 基金项目： | 北京市科技新星计划资助项目(2009B52) |
| 摘 要： | 采用微量肉汤稀释法和D-试验法检测64株凝固酶阴性葡萄球菌（CNs）对10种抗菌药物的耐药性，并用PCR方法分别检测头孢西丁耐药菌株和红霉素耐药菌株中mecA基因以及erinA、ermB、ermC和msrA基因的携带情况。结果表明，所有菌株均对万古霉素和甲氧苄啶／磺胺甲恶唑敏感；泰妙菌素为耐药率（65．6％）最高的抗菌药物，其次是红霉素、氧氟沙星和β-内酰胺类药物。28株（43．8％）CNS对青霉素耐药，其中26株对头孢西丁耐药（MRS）并且均携带mecA基因。30株（46．9％）CNS对红霉素耐药，其中24株为MLSB耐药表型，主要由ermB基因介导。总之，兽医临床CNs分离株对常用抗菌药物的耐药性不同，mecA基因和ermB基因的携带分别是兽医临床MRS和MLSB表型产生的主要原因。 |
| 关 键 词： | 凝固酶阴性葡萄球菌 耐药性 MRS MLSB 耐药基因 |
Detection of resistant genes and antimicrobial susceptibility of coagulase-negative Staphylococci(CNS) isolated from veterinary clinic |
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| ZHANG Chun-ping,CHEN Hui-juan,SONG Li,NING Yi-bao,MA Li.Detection of resistant genes and antimicrobial susceptibility of coagulase-negative Staphylococci(CNS) isolated from veterinary clinic[J].Chinese Journal of Veterinary Science,2011,31(11). | |
| Authors: | ZHANG Chun-ping CHEN Hui-juan SONG Li NING Yi-bao MA Li |
| Institution: | ZHANG Chun-ping1,CHEN Hui-juan2,SONG Li1,NING Yi-bao1,MA Li3(1.China Institute of Veterinary Drug Control,Beijing 100081,China,2.Yangzhou University,Yangzhou,Jiangsu 225009,3.Luoyang Huizhong Animal Medicine CO.,LTD,Luoyang,Henan 471003,China) |
| Abstract: | In order to investigate antimicrobial susceptibility of coagulase-negative staphylococci(CNS) isolated from veterinary clinic and mechanisms of resistance to antibiotics in clinically regular use were studied,broth microdilution methods and D-testing were used to detect antimicrobial susceptibility of 64 CNS to ten antibiotics,mecA gene in cefoxitin-resistant isolates and ermA,ermB,ermC,msrA genes in erythromycin-resistant isolates were detected by PCR methods in our study.The results showed that all isolat... |
| Keywords: | coagulase-negative Staphylococci(CNS) antimicrobial susceptibility MRS MLSB resistant genes |
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