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| 拮抗尖孢镰刀茵的木霉菌筛选方法评估 | |
| 引用本文： | 熊敏,高必达,陈捷.拮抗尖孢镰刀茵的木霉菌筛选方法评估[J].中国生物防治,2011,27(4):498-503. |
| 作者姓名： | 熊敏 高必达 陈捷 |
| 作者单位： | 1. 湖南农业大学生物安全科技学院,长沙410128：;E海交通大学农业与生物学院／农业部都市农业南方重点开放实验室,上海200240 2. 湖南农业大学生物安全科技学院,长沙410128： 3. E海交通大学农业与生物学院／农业部都市农业南方重点开放实验室,上海,200240 |
| 基金项目： | 国家公益性行业计划(200903052,200903056); 国家农业成果转化资金项目(2010GB2C00146); 上海市科委重点项目(09391910900) |
| 摘 要： | 利用11株不同木霉菌株分别比较对峙培养法、病原菌存活率测定法、生防菌分泌物降解病原菌测定法、浸种抑菌萌发试验法及种苗接种活体筛选法对抑制黄瓜枯萎病尖孢镰刀菌Fusariumoxysporum的拮抗木霉菌的筛选效率。试验表明不同的筛选方法获得拮抗木霉菌菌株的结果差异较大，其中病原菌存活率测定法与种苗活体接种筛选法结果一致性较高，可推荐作为生防木霉菌株初筛的有效方法。 |
| 关 键 词： | 木霉菌 尖孢镰刀菌 筛选方法 生物防治 |
Evaluation on Screening Methods for Trichoderma spp.Antagonistic to Fusarium oxysporum |
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| XIONG Min,GAO Bi-da,CHEN Jie of Ministry of Agriculture,Shanghai Jiao Tong University,Shanghai ,China.Evaluation on Screening Methods for Trichoderma spp.Antagonistic to Fusarium oxysporum[J].Chinese Journal of Biological Control,2011,27(4):498-503. | |
| Authors: | XIONG Min GAO Bi-da CHEN Jie of Ministry of Agriculture Shanghai Jiao Tong University Shanghai China |
| Institution: | XIONG Min1,2,GAO Bi-da1,CHEN Jie2 (1.Biosafety Science and Technology College,Hunan Agricultural University,Changsha 410128,2.School of Agriculture and Biology,Key Laboratory of Urban Agriculture(South) of Ministry of Agriculture,Shanghai Jiao Tong University,Shanghai 200240,China) |
| Abstract: | In attempt to establish an high-throughput screening method of biocontrol Trichoderma strains against Fusarium oxysporum in cucumber,authors compared selection efficiency of five screening methods,that is dual-culture method,pathogen survival measurement after treatment with antagonistic microbe,degradation of pathogen's mycelium after treatment with secretion of biocontrol microbe,seed germination recovery test after target pathogen infected by biocontrol microbe and the screening of biocontrol microbe on ... |
| Keywords: | Trichoderma spp Fusarium oxysporum screening methods biocontrol |
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