| 转cry1Ab基因水稻和两种杀虫剂对非靶标天敌拟环纹豹蛛免疫影响的评价 |
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| 引用本文： | 田俊策,陈洋,李兆亮,彭于发,叶恭银.转cry1Ab基因水稻和两种杀虫剂对非靶标天敌拟环纹豹蛛免疫影响的评价[J].中国生物防治,2011(4). |
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| 作者姓名： | 田俊策 陈洋 李兆亮 彭于发 叶恭银 |
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| 作者单位： | 浙江大学昆虫科学研究所/水稻生物学国家重点实验室/农业部作物病虫分子生物学重点开放实验室;中国农业科学院植物保护研究所植物病虫生物学国家重点实验室; |
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| 基金项目： | 国家重大基础研究发展计划(973)(2007CB109202); 国家转基因生物培育重大专项(2008ZX08011-01) |
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| 摘 要： | 本文测定了田间转cry1Ab基因水稻(KMD1和KMD2)和两种杀虫剂(吡虫啉和氟虫腈)处理非转基因亲本秀水11后拟环纹豹蛛Pardosa pseudoannulata的免疫指标(血细胞总数、包囊能力和酚氧化酶活性)的变化,并以非转基因亲本秀水11无药剂处理的作为对照。研究结果表明,KMD1和KMD2对拟环纹豹蛛的3种免疫因子都没有显著影响;两种杀虫剂处理均导致拟环纹豹蛛的血细胞数量和包囊能力显著下降,酚氧化酶活性略低于对照,即对拟环纹豹蛛的免疫有负面影响。可见,非靶标天敌的免疫能力可考虑作为转基因水稻环境安全性评价的一个简单有效的指标。
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| 关 键 词： | 转基因水稻 拟环纹豹蛛 免疫指标 |
Assessment of Effect of Transgenic Rice with cry1Ab Gene and Two Insecticides on Immune of Non-target Natural Enemy,Pardosa pseudoannulata |
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| TIAN Jun-ce,CHEN Yang,LI Zhao-liang,PENG Yu-fa,YE Gong-yin.Assessment of Effect of Transgenic Rice with cry1Ab Gene and Two Insecticides on Immune of Non-target Natural Enemy,Pardosa pseudoannulata[J].Chinese Journal of Biological Control,2011(4). |
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| Authors: | TIAN Jun-ce CHEN Yang LI Zhao-liang PENG Yu-fa YE Gong-yin |
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| Institution: | TIAN Jun-ce1,CHEN Yang1,LI Zhao-liang1,PENG Yu-fa2,YE Gong-yin1(1.Institute of Insect Sciences,State Key Laboratory of Rice Biology,Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Zhejiang University,Hangzhou 310029,2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | The immune parameters(total number of hemocytes,encapsulation rate and phenoloxidase activity) of Pardosa pseudoannulata from the transgenic cry1Ab rice(KMD1 and KMD2) fields and non-transgenic parental rice variety(Xiushui 11) fields which were sprayed the insecticides(imidacloprid and fipronil) were measured and compared to those parameters measured in spiders collected from Xiushui 11 with no insecticide sprayed.Results indicated that there were no significant differences in the immune factor of the P.ps... |
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| Keywords: | transgenic rice Pardosa pseudoannulata immune factor |
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