| SD大鼠EGFR基因cDNA序列的分子克隆 |
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| 引用本文： | 章金涛,张明昊.SD大鼠EGFR基因cDNA序列的分子克隆[J].中国兽医学报,2011,31(11). |
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| 作者姓名： | 章金涛 张明昊 |
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| 作者单位： | 1. 郑州大学实验动物中心,河南郑州,450052
2. 河南中医学院基础医学院,河南郑州,450008 |
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| 基金项目： | 国家自然科学基金资助项目(31071923); 河南省重点科技攻关资助项目(082102310061) |
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| 摘 要： | 参考大鼠EGFR基因序列，用RT-PCR方法对SD大鼠EGFR基因cDNA序列进行克隆，获得了SD大鼠EGFR基因的全长4127bp的cDNA序列（GenBank登录号HM801042），其中CDS长度为3627bp，编码1209个氨基酸。SD大鼠与国外报道的大鼠、小鼠、牛、猴、人等5个物种的EGFR基因cDNA序列的同源性分别为99．5％、92．9％、78．4％、83．4％、80．2％，其编码蛋白的氨基酸序列同源性分别为99．6％、95．9％、86．6％、89．0％、90．5％。这一结果表明了EGFR基N在进化过程中具有较高的保守性。通过比较SD大鼠EGFR基因与GenBank数据库中发布的EGFR基因的cDNA序列，本试验发现了10个SNP位点，其中5个没有改变氨基酸残基的性质，这一研究结果为EGFR基因的SNPs数据库提供了新的信息。
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| 关 键 词： | 大鼠 EGFR基因 分子克隆 序列分析 |
Molecular cloning of cDNA sequences encoding EGFR gene in the SD rats |
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| ZHANG Jin-tao,ZHANG Ming-hao.Molecular cloning of cDNA sequences encoding EGFR gene in the SD rats[J].Chinese Journal of Veterinary Science,2011,31(11). |
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| Authors: | ZHANG Jin-tao ZHANG Ming-hao |
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| Institution: | ZHANG Jin-tao1,ZHANG Ming-hao2(1.Laboratory Animal Center,Zhengzhou University,Zhengzhou 450052,China,2.School of Basic Medicine,Henan College of Traditional Chinese Medcine,Zhengzhou 450008,China) |
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| Abstract: | This study was designed to clone and analyze the cDNA encoding EGFR from Kunming mouse.The RT-PCR method was developed to clone the EGFR gene cDNA.A full-length cDNA and CDS sequences of SD rat were 4 127 bp and 3 627 bp,which has been accepted by GenBank(Accession Number:HM801042).EGFR protein encoded by this gene was composed of 1 209 amino acid residues.The identities of cDNA sequences of EGFR gene were 99.5%,92.9%,78.4%,83.4% and 80.2% by homologous comparison among SD rat and other species,and in amino... |
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| Keywords: | rat EGFR gene molecular cloning sequence analysis |
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