| 坏死梭杆菌白细胞毒素研究进展 |
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| 引用本文： | 张秀华,陈立志,刘晓颖.坏死梭杆菌白细胞毒素研究进展[J].动物医学进展,2007,28(9):74-77. |
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| 作者姓名： | 张秀华 陈立志 刘晓颖 |
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| 作者单位： | 中国农业科学院特产研究所,吉林,132109 |
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| 基金项目： | 吉林市科技发展计划
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中国农业科学院特产研究所科研启动基金 |
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| 摘 要： | 坏死梭杆菌是动物和人的各种坏死化脓感染的条件性致病菌.坏死梭杆菌的白细胞毒素是一种高度不稳定性分泌蛋白,被认为是主要的毒力因子.坏死梭杆菌白细胞毒素基因的开放阅读框(lktAORF)包括9 726 bp,编码3 241个氨基酸,总分子质量为336 ku的蛋白,且与其他细菌的细胞毒素没有任何相似的序列.覆盖在整个坏死梭杆菌lktA ORF上的5个短的重叠的多肽分别是BSBSE,SX,GAS,SH和FINAL,将它们在大肠埃希菌中表达,所有的多肽都有免疫原性,但GAS引起最小的抗体反应,BSBSE和SH对坏死梭杆菌攻击诱导产生了很强的保护力,比坏死梭杆菌的培养上清内全长活性lkt或无活性上清的保护性要好得多.
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| 关 键 词： | 坏死梭杆菌 白细胞毒素 毒力因子 |
| 文章编号： | 1007-5038（2007）09-0074-04 |
| 修稿时间： | 2007-04-20 |
Progress on Fusobacterium necrophorum Leukotoxin |
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| ZHANG Xiu-hua,CHEN Li-zhi,LIU Xiao-ying.Progress on Fusobacterium necrophorum Leukotoxin[J].Progress In Veterinary Medicine,2007,28(9):74-77. |
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| Authors: | ZHANG Xiu-hua CHEN Li-zhi LIU Xiao-ying |
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| Institution: | Institute of Special Economic Animal and Plant Science ,CAAS,Jilin,132109,China |
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| Abstract: | Fusobacterium necrophorum is generally an opportunistic pathogen by causing a wide variety of necrotic infections in animals and humans.Leukotoxin,a secreted protein,is a major virulence factor.The leukotoxin gene open reading frame(ORF;lktA) consists of 9 726 bp and encodes a protein of 3 241 amino acids with an overall molecular weight of 335 956.The leulotoxin have no sequence similarity with any other bacterial leukotoxin.The five overlapping leukotoxin gene truncations(BSBSE,SX,GAS,SH and FINAL) were expressed in E.coli.All polypeptides were immunogenic of which GAS polypeptide induces the least antibody response.Two polypeptides(BSBSE and SH) induced significant protection in mice against F.necrophorum infection,better than the full-length native leukotoxin or inactivated supernatant. |
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| Keywords: | Fusobacterium necrophorum leukotoxin virulence factor |
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